Micro-method for the qualitative and quantitative analysis of photosynthetic pigments using high-performance liquid chromatography
Micro-method for the qualitative and quantitative analysis of photosynthetic pigments using high-performance liquid chromatography
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高效液相色谱定性定量分析光合色素的微量方法
DOI：
10.1016/s0021-9673(00)98484-x
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发表时间：
1978
影响因子：
4.1
通讯作者：
M. Shiraki
中科院分区：
文献类型：
--
作者：
K. Iriyama;M. Yoshiura;M. Shiraki
材料和方法所有实验均在室温、完全黑暗或昏暗绿光下进行。菠菜叶（100 克鲜重）在 Warling 搅拌机中用 500 毫升丙酮均质化 3 分钟。将获得的青汁通过棉垫过滤以除去粗碎物，并将滤液在10，000g下离心5分钟以除去不溶物。根据 Iriyama 等人 aL5* 6 的方法，使用深绿色上清液（丙酮提取物）制备叶绿素 a 和 6。根据 Sievers 和 Hynninen 14 的方法，叶绿素 a' 和 6' 是通过糖柱上叶绿素 a 和 b 的再分级获得的。脱镁叶绿素a和b按照Hynninen15的方法制备。#&
MATERIALS AND METHODSAll the experiments were performed at room temperature in total darkness or under dim green light.Spinach leaves (100 g fresh weight) were homogenized for 3 min in a Warling blender with 500 ml of acetone. The green juice obtained was filtered through a pad of cotton to remove coarse debris, and the filtrate was centrifuged at 10, OOOg for 5 min to remove insoluble materials. The deep green supematant solution (acetone extract) was used to prepare chlorophylls a and 6, according to the method of Iriyama et aL5* 6. Chlorophylls a’and 6’were obtained by re-fractionation of chlorophylls a and b on a sugar column, according to the method of Sievers and Hynninen14. Pheophytins a and b were prepared according to the method of Hynninen15.# &