Short term signaling responses in roots of young soybean seedlings exposed to cadmium stress
Short term signaling responses in roots of young soybean seedlings exposed to cadmium stress
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DOI：
10.1016/j.jplph.2013.06.019
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发表时间：
2013-12-15
影响因子：
4.3
通讯作者：
Deckert, Joanna
中科院分区：
文献类型：
--
作者：
Chmielowska-Bak, Jagna;Lefevre, Isabelle;Deckert, Joanna
在本研究中，研究了大豆幼苗（Glycine max）在短时间（3,6和24 h）暴露于两种浓度镉（10 mg L-1和25 mg L-1）下14个参与各种信号转导途径的基因的表达。结果表明，镉诱导了参与乙烯和多胺代谢、一氧化氮生成、MAPK级联反应和其他基因表达调控的蛋白质编码基因。对cd诱导基因启动子序列的生物信息学分析表明，cd诱导基因启动子具有多个与植物对胁迫因子的响应、脱落酸和乙烯信号通路相关的调控基序。通过实时分析CdCl2处理24 h的乙烯产量，进一步证实了乙烯参与大豆幼苗对镉胁迫的响应。讨论了上述信号元件在大豆幼苗镉信号转导中的作用。(C) 2013爱思唯尔有限公司版权所有。
In the present study, the expression of fourteen genes involved in various signal transduction pathways was examined in young soybean (Glycine max) seedlings exposed to cadmium at two concentrations (10 mg L-1 and 25 mg L-1) for short time periods (3, 6 and 24 h). The results show that cadmium causes induction of genes encoding proteins involved in ethylene and polyamines metabolism, nitric oxide generation, MAPK cascades and regulation of other genes' expression. The bioinformatic analysis of promoter sequences of Cd-inducible genes revealed that their promoters possess several regulative motifs associated with the plant response to stress factors and abscisic acid and ethylene signaling. The involvement of ethylene in the response of soybean seedlings to cadmium stress was further confirmed by the realtime analysis of ethylene production during 24 h of CdCl2 treatment. The role of the described signaling elements in transduction of the cadmium signal in young soybean seedlings is discussed. (C) 2013 Elsevier GmbH. All rights reserved.