Permeability of cerebral blood vessels in experimental allergic encephalomyelitis studied by radioactive iodinated bovine albumin
Permeability of cerebral blood vessels in experimental allergic encephalomyelitis studied by radioactive iodinated bovine albumin
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放射性碘化牛白蛋白研究实验性变应性脑脊髓炎脑血管通透性
DOI：
10.1212/wnl.10.2.171
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发表时间：
1960
期刊：
影响因子：
9.9
通讯作者：
B. Rozdilsky
中科院分区：
文献类型：
--
作者：
M. Vulpe;A. Hawkins;B. Rozdilsky
这些实验的目的是研究实验性过敏性脑脊髓炎（EAE）发展过程中神经系统的血管通透性以及通透性变化与该疾病组织学病变的时间和空间关系。这一信息有望揭示该疾病发病机制的一个方面，即目前尚不清楚的原发病变部位。如果它位于血管壁，则在看到其他炎症迹象之前，血管通透性可能会增加；如果是在周围的实质中，渗透性的增加可能与血管壁周围的炎症反应同时出现，或者可能稍晚一些。 Barlow2 使用台盼蓝研究了 EAE 中的脑血管通透性，发现血管周围浸润区域的通透性增加，但血管周围套囊区域的通透性没有增加。我们研究了碘化放射性牛白蛋白的渗透性，采用放射自显影方法进行检测。该方法比台盼蓝定位更精确，染色强度更高。此外，我们可以预期，对蛋白质通透性变化的研究可能与EAE的发病机制问题更加相关，EAE被认为是过敏反应的表现。
THE PURPOSE of these experiments was to study vascular permeability in the nervous system during the development of experimental allergic encephalomyelitis (EAE) and the temporal and spatial relationship of any changes in permeability to the histologic lesions of this disease. This information might be expected to shed light upon one aspect of the pathogenesis of this disease, namely the site of the primary lesion, which at present is not known. Should it be in the wall of the vessels, the vascular permeability might be increased before other signs of inflammation can be seen; should it be in the surrounding parenchyma, the increase in permeability could appear simultaneously with-or perhaps slightly later than-the inflammatory reaction around the vessel wall. Barlow2 has investigated cerebral vascular permeability in EAE, using trypan blue, and found increased permeability in regions with perivascular infiltration but not in those with perivascular cuffing. We have studied the permeability with iodinated radioactive bovine albumin, employing an autoradiographic method for its detection. This method gives a more precise localization than trypan blue and a greater intensity of staining. In addition, we can expect that study of the changes of protein permeability may be more relevant to the problem of pathogenesis of EAE, which is considered to be a manifestation of a hypersensitive reaction.