Cloning and Tissue Expression of Chicken Heart Fatty Acid-Binding Protein and Intestine Fatty Acid-Binding Protein Genes
Cloning and Tissue Expression of Chicken Heart Fatty Acid-Binding Protein and Intestine Fatty Acid-Binding Protein Genes
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DOI：
10.1080/10495390500276882
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发表时间：
2005-01
影响因子：
3.7
通讯作者：
Qigui Wang;Hui Li;Shuang Liu;Guihua Wang;Yu-xiang Wang
中科院分区：
文献类型：
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作者：
Qigui Wang;Hui Li;Shuang Liu;Guihua Wang;Yu-xiang Wang
脂肪酸结合蛋白（Fatty Acid-binding Proteins，FABPs）是一个脂质结合蛋白超家族的成员，存在于无脊椎动物和脊椎动物的细胞内。本研究旨在克隆鸡心脏脂肪酸结合蛋白和肠脂肪酸结合蛋白的基因，并对其进行功能鉴定。根据鸡EST序列设计引物，从鸡心和肠组织中扩增出H-FABP和I-FABP基因的cDNA。序列分析表明，鸡H-FABP基因的cDNA与人、小鼠和猪H-FABP基因的同源性为75 - 77%，鸡I-FABP基因与人、小鼠和猪I-FABP基因的同源性为71 - 72%。北方印迹分析表明，H-FABP基因在多种组织中表达，而I-FABP基因仅在肠组织中表达，与哺乳动物的伴侣基因相似。6周龄时，肥系肉鸡和白二鸡心脏H-FABP mRNA和肠道I-FABP mRNA的表达量存在显著差异。本研究结果为研究两种脂肪酸结合蛋白在禽类脂肪酸代谢调控中的作用提供了分子基础信息。
Fatty acid-binding proteins (FABPs) are members of a superfamily of lipid-binding proteins, occurring intracellularly in invertebrates and vertebrates. This study was designed to clone and characterize the genes of heart fatty acid-binding protein and intestine fatty acid-binding protein in the chicken. PCR primers were designed according to the chicken EST sequences to amplify cDNA of H-FABP and I-FABP genes from chicken heart and intestinal tissues. Analysis of sequence showed that the cDNA of the chicken H-FABP gene is 75 to 77% homologues to human, mouse, and pig H-FABP genes, and the chicken I-FABP gene is 71 to 72% homologues to human, mouse, and pig I-FABP genes. In addition, Northern blot analysis indicated that of the two genes, similar to the copartner of the mammal, H-FABP gene was expressed in a wide variety of tissues, and I-FABP gene was expressed only in intestinal tissues. The expression levels of the chicken H-FABP mRNA in heart and I-FABP mRNA in intestine had significant differences between the broilers from fat line and Bai'er layers at six weeks of age. The results of this study provided basic molecular information for studying the role of two FABPs in the regulation of fatty acid metabolism in avian species.