Amino Acid-Derived Sensors for Specific Zn2+ Detection Using Hyperpolarized 13 C Magnetic Resonance Spectroscopy.
Amino Acid-Derived Sensors for Specific Zn2+ Detection Using Hyperpolarized 13 C Magnetic Resonance Spectroscopy.
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使用超极化 13 C 磁共振波谱进行特定 Zn2 检测的氨基酸衍生传感器。
DOI：
10.1002/chem.201902771
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发表时间：
2019
期刊：
影响因子：
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通讯作者：
Flavell,RobertR
中科院分区：
文献类型：
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作者：
Wang,Sinan;Korenchan,DavidE;Perez,PaolaM;Taglang,Céline;Hayes,ThomasR;Sriram,Renuka;Bok,Robert;Hong,AndrewS;Wu,Yunkou;Li,Henry;Wang,Zhen;Kurhanewicz,John;Wilson,DavidM;Flavell,RobertR
在正常组织和疾病状态中观察到Zn2+浓度的变化，因此Zn2+的成像是一个活跃的研究领域。在本文中，使用超极化13 C磁共振光谱法开发了富集的[1 - 13 C]半胱氨酸和[1 - 13 C2]亚氨基二乙酸作为Zn2+特异性成像探针。[1 - 13 C]半胱氨酸用于准确定量复杂生物混合物中的Zn2+。这些传感器可以用来检测Zn2+通过成像机制，包括在13 C化学位移，共振线宽，或T1的变化。
Alterations in Zn2+concentration are seen in normal tissues and in disease states, and for this reason imaging of Zn2+is an area of active investigation. Herein, enriched [1‐13C]cysteine and [1‐13C2]iminodiacetic acid were developed as Zn2+‐specific imaging probes using hyperpolarized13C magnetic resonance spectroscopy. [1‐13C]cysteine was used to accurately quantify Zn2+in complex biological mixtures. These sensors can be employed to detect Zn2+via imaging mechanisms including changes in13C chemical shift, resonance linewidth, orT1.