Plasmodium diversity in non-malaria individuals from the Bioko Island in Equatorial Guinea (West Central-Africa).
Plasmodium diversity in non-malaria individuals from the Bioko Island in Equatorial Guinea (West Central-Africa).
复制标题
DOI：
10.1186/1476-072x-5-27
复制
发表时间：
2006-06-19
影响因子：
4.9
通讯作者：
Llanes AB
中科院分区：
文献类型：
--
作者：
Guerra-Neira A;Rubio JM;Royo JR;Ortega JC;Auñón AS;Diaz PB;Llanes AB
本文对疟原虫进行了分析。在比奥科岛(赤道几内亚)，疟疾是一种高度地方性疾病，隔离状态不同的三个村庄的流行率。我们还利用几种表现出高度多态的恶性疟原虫蛋白(MSP-1和MSP-2)作为标记，描述了恶性疟原虫的遗传多样性。对从三个不同人群获得的结果进行了比较，以确定人类活动和干预的影响。疟原虫sp.对比奥科岛(赤道几内亚)的三个村庄进行了分析，其中一个村庄(南部)因地理障碍而与世隔绝。采用半套式多重聚合酶链式反应(PCR)技术检测四种人疟原虫的流行情况。通过聚合酶链式反应检测裂殖子表面蛋白1和2基因(MSP-1和MSP-2)的目标多态区域，确定恶性疟原虫群体的基因分型和频率。所获得的数据表明，在不同种类的疟原虫流行方面，西北地区和东部地区的疟原虫种群流动没有差异。另一方面，南方人群显示出少量的疟原虫和较高的卵形疟原虫的流行率，这表明某种类型的传播是与其他两个分离的。不同地区的恶性疟原虫基因分型表明，比奥科岛的寄生虫种群具有相当大的等位基因多样性，尽管南部地区的等位基因多样性略高于其他地区。寄生虫血症水平与感染多样性(MOI)个体的年龄有关。结果可以通过特定的MSP等位基因的选择来解释。这往往会限制寄生虫种群的多样性，并导致稀有等位基因的灭绝。另一方面，隔离村内寄生虫种群受外界影响较小，维持较高的恶性疟原虫多样性。了解寄生虫种群及其关系对于研究其对控制干预的影响是必要的。
In this paper we analyse the Plasmodium sp. prevalence in three villages with different isolation status on the island of Bioko (Equatorial Guinea) where malaria is a hyper-endemic disease. We also describe the genetic diversity of P. falciparum, using several plasmodia proteins as markers which show a high degree of polymorphism (MSP-1 and MSP-2). The results obtained from three different populations are compared in order to establish the impact of human movements and interventions. Plasmodium sp. were analysed in three villages on Bioko Island (Equatorial Guinea), one of which (Southern) is isolated by geographical barriers. The semi-nested multiplex polymerase chain reaction (PCR) technique was used to determine the prevalence of the four human plasmodia species. The genotyping and frequency of P. falciparum populations were determined by PCR assay target polymorphism regions of the merozoite surface proteins 1 and 2 genes (MSP-1 and MSP-2). The data obtained show that there are no differences in plasmodia population flow between the Northwest and Eastern regions as regards the prevalence of the different Plasmodium species. The Southern population, on the other hand, shows a minor presence of P. malariae and a higher prevalence of P. ovale, suggesting some kind of transmission isolated from the other two. The P. falciparum genotyping in the different regions points to a considerable allelic diversity in the parasite population on Bioko Island, although this is somewhat higher in the Southern region than the others. There was a correlation between parasitaemia levels and the age of the individual with the multiplicity of infection (MOI). Results could be explained by the selection of particular MSP alleles. This would tend to limit diversity in the parasite population and leading up to the extinction of rare alleles. On the other hand, the parasite population in the isolated village has less outside influence and the diversity of P. falciparum is maintained higher. The knowledge of parasite populations and their relationships is necessary to study their implications for control intervention.