Effect of vitrification on promoter methylation and the expression of pluripotency and differentiation genes in mouse blastocysts
Effect of vitrification on promoter methylation and the expression of pluripotency and differentiation genes in mouse blastocysts
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DOI：
10.1002/mrd.22052
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发表时间：
2012-07
影响因子：
2.5
通讯作者：
Xueming Zhao;W. Du;H. Hao;Dong Wang;T. Qin;Yan Liu;Huabin Zhu
中科院分区：
文献类型：
--
作者：
Xueming Zhao;W. Du;H. Hao;Dong Wang;T. Qin;Yan Liu;Huabin Zhu
The present study was designed to determine the effects of vitrification on promoter methylation and the expression levels of pluripotency and differentiation genes in mouse blastocysts. Promoter region CpG methylation patterns and the expression levels of octamer‐binding transcription factor (Oct4), Nanog homeobox (Nanog), caudal‐type homeobox 2 (Cdx2), and heart and neural crest derivatives‐expressed transcript 1 (Hand1) were analyzed in fresh and vitrified mouse blastocysts. Methylation was measured by bisulphate mutagenesis and sequencing; gene expression was determined by real‐time reverse transcription‐PCR. The results showed that vitrification significantly reduced the methylation levels of the Oct4 (85% vs. 62.5%), Nanog (77.5% vs. 55%), and Cdx2 promoters (4.6% vs. 1.4%; P 0.05). The expression levels of Cdx2 and Hand1 were not significantly different in fresh and vitrified blastocysts. In conclusion, vitrification significantly decreased Oct4, Nanog, and Cdx2 promoter methylation in mouse blastocysts, which correlated with increased expression of Oct4 and Nanog. Mol. Reprod. Dev. 79:445–450, 2012. © Wiley Periodicals, Inc.
