Localization of septin 8 in murine retina, and spatiotemporal expression of septin 8 in a murine model of photoreceptor cell degeneration
Localization of septin 8 in murine retina, and spatiotemporal expression of septin 8 in a murine model of photoreceptor cell degeneration
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DOI：
10.1016/j.neulet.2007.07.030
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发表时间：
2007-08-23
影响因子：
2.5
通讯作者：
Mori, Hideki
中科院分区：
文献类型：
--
作者：
Hara, Akira;Taguchi, Ayako;Mori, Hideki
septin 是哺乳动物中细胞骨架 GTP 结合蛋白的保守家族，包含稳定的异聚复合物，并在细胞分裂后的蛋白质支架、胞质分裂、囊泡运输和质膜完整性中发挥多种作用。本研究的目的是确定 septin 8 在小鼠成年视网膜中的定位，并分析 septin 8 在小鼠感光细胞变性模型中的时空表达。采用免疫组化和Western blotting方法观察小鼠和大鼠正常视网膜中septin 8的表达。此外，通过免疫组织化学结合苏木精和伊红染色以及原位DNA片段标记方法检测了小鼠感光细胞变性中septin 8表达的时间过程。在正常小鼠和大鼠视网膜中，septin 8 的定位仅限于感光细胞的细胞核。玻璃体内注射氯化钴后 96 小时，大多数感光细胞在核 DNA 断裂的同时失去了 septin 8 免疫染色。这项研究的结果表明，septin 8 蛋白存在于视网膜内的特定位置。此外，感光细胞核中 Septin 8 的消失伴随着核 DNA 断裂。这表明 Septin 8 的缺失可能是感光细胞变性的有用预后指标。 (C) 2007 Elsevier Ireland Ltd. 保留所有权利。
The septins, which form a conserved family of cytoskeletal GTP-binding proteins in mammals, comprise stable heteromeric complexes and have diverse roles in protein scaffolding, cytokinesis, vesicle trafficking and plasma membrane integrity following cell division. The goal of this study was to determine the localization of septin 8 in murine adult retina, and analyze the spatiotemporal expression of septin 8 in a murine model of photoreceptor cell degeneration. Expression of septin 8 in the normal retina of mouse and rat was observed by using immunohistochemistry and Western blotting. Furthermore, time course of the expression of septin 8 in mouse photoreceptor cell degeneration were examined by immunohistochemistry combined with hematoxylin and eosin staining, and in situ DNA fragment labeling method. In normal mouse and rat retina, localization of septin 8 is restricted in nuclei of photoreceptor cells. 96 h after intravitreal injection of cobalt chloride most photoreceptor cells lost septin 8 immunostaining at the same time as nuclear DNA fragmentation. The results of this study show that septin 8 protein is present in the specific location within the retina. Furthermore, the disappearance of septin 8 in the nuclei of photoreceptor cells is concomitant with nuclear DNA fragmentation. This suggests that loss of septin 8 could be a useful prognostic indicator for photoreceptor cell degeneration. (C) 2007 Elsevier Ireland Ltd. All rights reserved.