Automatic quantification of morphological traits via three-dimensional measurement of Arabidopsis
Automatic quantification of morphological traits via three-dimensional measurement of Arabidopsis
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DOI：
10.1111/j.1365-313x.2004.02042.x
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发表时间：
2004-04-01
期刊：
影响因子：
7.2
通讯作者：
Toyoda, T
中科院分区：
文献类型：
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作者：
Kaminuma, E;Heida, N;Toyoda, T
许多突变体已经从模式植物拟南芥中分离出来，最近重要的遗传资源，如T-DNA敲除系，促进了鉴定新突变体的速度。然而，目前突变体筛选的表型分析主要依赖于形态学性状目测后的定性描述。我们提出了一种基于激光测距仪（LRF）精确三维测量和自动数据处理的表型分析新方法。通过对2个拟南芥生态型幼苗的三维表面测量，成功定量定义了叶片表面方向和叶片伸长两个新性状。与传统的二维测量相比，所提出的方法使我们能够获得定量和精确的植物形态描述。该方法将为基于3D数据从突变池或自然生态型中发现新性状开辟一条道路。
Many mutants have been isolated from the model plant Arabidopsis thaliana, and recent important genetic resources, such as T-DNA knockout lines, facilitate the speed of identifying new mutants. However, present phenotypic analysis of mutant screens depends mainly on qualitative descriptions after visual observation of morphological traits. We propose a novel method of phenotypic analysis based on precise three-dimensional (3D) measurement by a laser range finder (LRF) and automatic data processing. We measured the 3D surfaces of young plants of two Arabidopsis ecotypes and successfully defined two new traits, the direction of the blade surface and epinasty of the blade, quantitatively. The proposed method enables us to obtain quantitative and precise descriptions of plant morphologies compared to conventional 2D measurement. The method will open a way to find new traits from mutant pools or natural ecotypes based on 3D data.