Effects of fish oil on the neuro-endocrine responses to an endotoxin challenge in healthy volunteers
Effects of fish oil on the neuro-endocrine responses to an endotoxin challenge in healthy volunteers
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DOI：
10.1016/j.clnu.2006.06.001
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发表时间：
2007-02-01
影响因子：
6.3
通讯作者：
Chiolero, Rene
中科院分区：
文献类型：
--
作者：
Michaeli, Burkhard;Berger, Mette M.;Chiolero, Rene
背景与目的：鱼油（FO）已被证明可以调节急性和慢性炎症反应。内毒素（LPS）已被证明模拟脓毒症的几个方面。本研究旨在测试口服FO补充剂对静脉注射LPS的健康受试者的全身和内分泌反应的影响。受试者和方法：15名健康男性（年龄26.0 ± 3.1岁，BMI 23.8 ± 1.9 kg /m2）入组。受试者随机接受3-4周的口服FO补充（7.2 g/天，提供1.1 g/天的20：5（n-3）和0.7 g/天的22：6（n-3）脂肪酸）或不补充，然后接受内毒素挑战：2 ng/kg LPS。所有受试者均接受两次研究（安慰剂和LPS）。测量：至关重要。症状，能量消耗（EE），葡萄糖和脂质代谢（（2）H（2）-葡萄糖），血浆细胞因子和应激激素为6小时后LPS或安慰剂。结果：LPS引起细胞因子释放，发热，增加心率，静息EE和底物氧化，血浆胰高血糖素和葡萄糖浓度;神经内分泌反应的特点是增加血浆应激激素。FO显著减弱发热、ACTH和皮质醇。血浆水平（对细胞因子释放无影响）。结论：FO补充可减弱LPS后内分泌应激反应和体温升高，但对LPS后细胞因子的产生无影响。这些发现与FO对花生四烯酸代谢和细胞因子释放的假定抗炎作用相冲突。这些结果表明，FO可能在脓毒症中发挥作用，但无炎症，需要进一步研究（c）2006年Elsevier Ltd和欧洲临床营养与代谢学会保留。
Background Et aims: Fish oil (FO) has been shown to modulate the acute and chronic inflammatory responses. Endotoxin (LPS) has been shown to mimic several aspects of sepsis. The study aimed at testing the effects of oral FO supplements in healthy subjects submitted to intravenous LPS on systemic and endocrine response.Subjects and methods: Fifteen healthy men (aged 26.0 +/- 3.1 years, BMI 23.8 +/- 1.9 kg /m(2)), were enrolled. Subjects were randomised to 3-4 weeks of oral FO supplementation (7.2 g/ day, providing 1.1 g/day of 20:5(n-3) and 0.7 g/day of 22:6 (n-3) fatty acids) or no supplementation and then submitted to endotoxin challenge: 2 ng/kg of LPS. All subjects were studied twice (placebo and LPS). Measurements: vital. signs, energy expenditure (EE), glucose and lipid metabolism ((2)H(2)-glucose), plasma cytokines and stress hormones for 6 h after LPS or placebo.Results: LPS caused cytokine release, fever, increases in heart rate, resting EE and substrate oxidation, plasma glucagon and glucose concentrations; the neuro-endocrine response was characterised by increased plasma stress hormones. FO significantly blunted fever, ACTH and cortisol. plasma levels (no effect on cytokine release). FO blunted the peak norepinephrine after LPS.Conclusion: FO supplements blunted the endocrine stress response and the increase in body temperature, but had no impact on cytokine production after LPS. These findings conflict with the postulated anti-inflammatory effects of FO on arachidonic acid metabolism and cytokine release. These results suggest that FO may exert effects in sepsis though non-inflammatory which require further investigations (c) 2006 Elsevier Ltd and European Society for Clinical Nutrition and Metabolism reserved.