Radical Scavenging Capacity of Methanolic Phillyrea latifolia L. Extract: Anthocyanin and Phenolic Acids Composition of Fruits
Radical Scavenging Capacity of Methanolic Phillyrea latifolia L. Extract: Anthocyanin and Phenolic Acids Composition of Fruits
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DOI：
10.3390/molecules18021798
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发表时间：
2013-02-01
期刊：
影响因子：
4.6
通讯作者：
Erkan, Naciye
中科院分区：
文献类型：
--
作者：
Ayranci, Erol;Erkan, Naciye
参考花青素标准品（类黄酮）和酚酸标准品（苯丙素），研究了来自 Phillyrea latifolia L.（俗称绿橄榄树或假女贞）果实的粗甲醇提取物的自由基清除能力。高效液相色谱-二极管阵列检测 (HPLC-DAD) 表征表明，角花青素、黑曼素、花青素、阿魏酸、咖啡酸和迷迭香酸的含量分别为 289.1、90.4、191.4、225.2、221.2 和 190.1 mg/100 g 水果鲜重 (FW)。发现绿原酸和对香豆酸的含量较低。植物提取物的 Trolox 当量抗氧化能力 (TEAC) 和 IC50 值分别为 1.8 mM Trolox 当量 (TE)/g FW 水果和 69.4 μg/mL，表明提取物的自由基清除活性与角花青素和对香豆酸接近。宽叶松果的粗甲醇提取物被认为具有相当有效的自由基清除作用。植物提取物的总酚含量 (TPC) 为 1652.9 毫克没食子酸当量 (GAE)/100 克 FW 水果。
Radical scavenging capacity of a crude methanolic extract from the fruits of Phillyrea latifolia L., commonly known as green olive tree or mock privet, was investigated with reference to anthocyanin standards, as flavonoids, and phenolic acid standards, as phenylpropanoids. Characterization with high performance liquid chromatography-diode array detection (HPLC-DAD) indicated the presence of keracyanin, kuromanin, cyanidin, ferulic acid, caffeic acid and rosmarinic acid at amounts of 289.1, 90.4, 191.4, 225.2, 221.2 and 190.1 mg/100 g fresh weight (FW) of fruits, respectively. Chlorogenic and p-coumaric acids were found to exist in lower amounts. Trolox equivalent antioxidant capacity (TEAC) and IC50 values of the plant extract were found to be 1.8 mM Trolox equivalents (TE)/g FW of fruits and 69.4 mu g/mL, respectively, indicating the close radical scavenging activity of the extract to those of keracyanin and p-coumaric acid. The crude methanolic P. latifolia L. fruit extract was seen to be fairly potent in radical scavenging. Total phenolic content (TPC) of the plant extract was found to be 1652.9 mg gallic acid equivalent (GAE)/100 g FW of fruits.