New technologies accelerate the exploration of non-coding RNAs in horticultural plants.
New technologies accelerate the exploration of non-coding RNAs in horticultural plants.
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DOI：
10.1038/hortres.2017.31
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发表时间：
2017
影响因子：
8.7
通讯作者：
Yang X
中科院分区：
文献类型：
--
作者：
Liu D;Mewalal R;Hu R;Tuskan GA;Yang X
非编码RNA（ncRNA），即不翻译成蛋白质的RNA，是植物中多种生物学过程的关键调节因子。虽然蛋白质编码基因在测序的基因组中已经得到了相对良好的注释，占植物基因组空间的一小部分，但植物ncRNA的范围正在迅速扩大。实验和计算技术的最新进展为ncRNA的发现和功能表征产生了巨大的动力。本文综述了植物ncRNA的分类和已知的生物学功能，综述了新一代测序（NGS）技术和核糖体分析技术在园艺植物ncRNA发现中的应用，并讨论了新技术的应用，特别是新的基因组编辑工具定期间隔短回文重复序列（CRISPR）/CRISPR相关蛋白9（Cas9）系统，植物ncRNA的功能表征。
Non-coding RNAs (ncRNAs), that is, RNAs not translated into proteins, are crucial regulators of a variety of biological processes in plants. While protein-encoding genes have been relatively well-annotated in sequenced genomes, accounting for a small portion of the genome space in plants, the universe of plant ncRNAs is rapidly expanding. Recent advances in experimental and computational technologies have generated a great momentum for discovery and functional characterization of ncRNAs. Here we summarize the classification and known biological functions of plant ncRNAs, review the application of next-generation sequencing (NGS) technology and ribosome profiling technology to ncRNA discovery in horticultural plants and discuss the application of new technologies, especially the new genome-editing tool clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 9 (Cas9) systems, to functional characterization of plant ncRNAs.