Microtubule changes and cytotoxicity in leukemic cell lines treated with taxol.
Microtubule changes and cytotoxicity in leukemic cell lines treated with taxol.
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发表时间：
1988-07
期刊：
影响因子：
11.2
通讯作者：
E. Rowinsky;R. Donehower;R. J. Jones;R. Tucker
中科院分区：
文献类型：
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作者：
E. Rowinsky;R. Donehower;R. J. Jones;R. Tucker
紫杉醇是一种能促进微管蛋白聚合的二萜类植物产品，目前正在进入治疗人类白血病的临床试验。为了开发一种预测肿瘤对紫杉醇敏感性的体外试验，将人白血病细胞系暴露于临床可达到浓度的紫杉醇并持续相关暴露时间。通过间接免疫荧光观察到的微管变化与通过克隆形成试验测量的药物敏感性进行比较。紫杉醇在整个细胞周期中产生G2/M期的多个有丝分裂星状体或微管集束。在对紫杉醇相对耐药的细胞中，微管集束是可逆的，而在相对敏感的细胞中，微管集束在存在或不存在紫杉醇的情况下持续存在。相比之下，在任何细胞系中，紫菀形成与细胞毒性无关。将来，这些微管效应可能有助于预测紫杉醇的化疗疗效。
Taxol, a diterpenoid plant product that enhances the polymerization of tubulin, is currently entering clinical trials in the treatment of human leukemia. In order to develop an in vitro assay to predict tumor sensitivity to taxol, human leukemic cell lines were exposed to clinically achievable concentrations of taxol for relevant exposure periods. Changes in microtubules visualized by indirect immunofluorescence were compared to drug sensitivity measured by a clonogenic assay. Taxol produced either multiple mitotic asters in G2/M or microtubule bundling throughout the cell cycle. In cells that were relatively resistant to taxol, microtubule bundling was reversible while microtubule bundling in relatively sensitive cells persisted in the presence or absence of taxol. In contrast, aster formation was unrelated to cytotoxicity in any cell line. In the future, these microtubule effects may be useful in predicting the chemotherapeutic efficacy of taxol.