Identification of novel members of G-protein coupled receptor superfamily expressed in bovine taste tissue.
Identification of novel members of G-protein coupled receptor superfamily expressed in bovine taste tissue.
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牛味觉组织中表达的 G 蛋白偶联受体超家族新成员的鉴定。
DOI：
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发表时间：
1993
期刊：
影响因子：
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通讯作者：
K. Kurihara
中科院分区：
文献类型：
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作者：
I. Matsuoka;T. Mori;J. Aoki;T. Sato;K. Kurihara
利用逆转录-聚合酶链反应（RT-PCR）和简并寡核苷酸引物，从牛味觉组织中扩增出G蛋白偶联受体（GCR）超家族的两个不同亚家族的新成员。由多个cDNA克隆组成的A型受体克隆与推测的嗅觉受体亚家族有显著的相似性，而单个B型受体克隆与肽受体亚家族有显著的相似性。这些受体在味觉细胞的生理功能进行了讨论。
Using reverse transcription-polymerase chain reaction (RT-PCR) and degenerate oligonucleotide primers, we amplified novel members of two different subfamily of G-protein coupled receptor (GCR) superfamily from bovine taste tissue. Type A receptor clones composed of multiple cDNA clones had significant similarity with putative olfactory receptor subfamily, while a single type B clone had significant similarity with peptide receptor subfamily. Physiological functions of these receptors in taste cells are discussed.