Tandem mass spectrometry applied to protein structure problems.
Tandem mass spectrometry applied to protein structure problems.
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串联质谱法应用于蛋白质结构问题。
DOI：
10.1042/bst0170237
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发表时间：
1989
影响因子：
3.9
通讯作者：
Biemann,K
中科院分区：
文献类型：
--
作者：
Biemann,K
随着场脱附[11]、等离子体脱附[2]和快速原子轰击（FAB）[3]电离技术的发展，用质谱法电离大分子和极性分子，从而测定其分子质量已经成为可能。FAB尤其容易使用，因为它简单、直接，易于适应最广泛使用的质谱仪，即四极杆和磁偏转仪器。后者尤其具有高质量范围和分辨率来分析FAB-ms产生的离子。这种情况导致商用磁性仪器的质量范围扩展到质量10,000甚至更高，在全加速电压下，因此具有全灵敏度。
With the development of field desorption [11, plasma desorption [2] and fast atom bombardment (FAB)[3] ionization, it has become possible to ionize large and polar molecules and thus determine their molecular masses by ms Particularly easy to use is FAB because it is simple, straightforward and easily adaptable to the most widely available mass spectrometers, namely quadrupole and magnetic deflection instruments. The latter especially have the high mass range and resolution to analyse the ions generated by FAB-ms This situation led to the extension of the mass range of commercially available magnetic instruments to mass 10 000 and beyond, at full accelerating voltage and thus full sensitivity.