Allokutzneria multivorans sp. nov., an actinomycete isolated from soil.
Allokutzneria multivorans sp. nov., an actinomycete isolated from soil.
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DOI：
10.1099/ijs.0.054411-0
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发表时间：
2013-11
影响因子：
2.8
通讯作者：
Yan-Ru Cao;Xiu Chen;Yi Jiang;Lisong Wang;Cheng-lin Jiang
中科院分区：
文献类型：
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作者：
Yan-Ru Cao;Xiu Chen;Yi Jiang;Lisong Wang;Cheng-lin Jiang
从云南怒江畔的土壤中分离到一株菌丝分枝良好的放线菌，命名为Yim 120521(T)。气生菌丝体和基生菌丝体均为白色，无色。在4-40°C(最适28°C)、pH 6.0-9.0(最适7.0)和0-4%(w/v)氯化钠(最适0%)条件下生长。16S rRNA基因序列分析表明，菌株Yim 120521(T)属于异形藻属，与Alkutzneria albata Dsm 44149(T)的同源性最高(98.4%)。然而，两个菌株之间的DNA-DNA关联度平均值在70%以下。化学分类特征支持菌株Yim 120521(T)属于异形藻属，全细胞糖为鼠李糖、阿拉伯糖、葡萄糖、半乳糖和甘露糖，胞壁二胺二酸为中位二胺二胺酸，主要为MK-9(H4)。根据生理生化和化学分类特征，菌株YIM 120521(T)被认为是异形藻属的一个新种，命名为多食异形藻。11月是提议的。模式株为YIM 120521(T)(=JCM 17342(T)=DSM 45532(T))。
An actinomycete with well-branched mycelia, designated strain YIM 120521(T), was isolated from soil collected from the banks of the Nujiang River, Yunnan Province, south-west China. Both aerial and substrate mycelia were white and non-pigmented. Growth was observed at 4-40 °C (optimum 28 °C), pH 6.0-9.0 (optimum 7.0) and in 0-4 % (w/v) NaCl (optimum 0 %). Analysis of the 16S rRNA gene sequence revealed that strain YIM 120521(T) belongs to the genus Allokutzneria with the highest sequence similarity to Allokutzneria albata DSM 44149(T) (98.4 %). However, the mean DNA-DNA relatedness value between the two strains was below 70 %. Chemotaxonomic characteristics supported the inclusion of strain YIM 120521(T) in the genus Allokutzneria, with rhamnose, arabinose, glucose, galactose and mannose as the whole-cell sugars, meso-diaminopimelic acid as the cell-wall diamino acid and MK-9(H4) as the predominant menaquinone. On the basis of physiological, biochemical and chemotaxonomic characteristics, strain YIM 120521(T) is considered to represent a novel species of the genus Allokutzneria, for which the name Allokutzneria multivorans sp. nov. is proposed. The type strain is YIM 120521(T) ( = JCM 17342(T) = DSM 45532(T)).