Femtosecond Laser-Induced Micropattern and Ca/P Deposition on Ti Implant Surface and Its Acceleration on Early Osseointegration
Femtosecond Laser-Induced Micropattern and Ca/P Deposition on Ti Implant Surface and Its Acceleration on Early Osseointegration
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飞秒激光诱导钛种植体表面微图案和 Ca/P 沉积及其对早期骨整合的加速
DOI：
10.1021/am402290e
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发表时间：
2013-08-28
影响因子：
9.5
通讯作者：
Yang, Xianjin
中科院分区：
文献类型：
--
作者：
Liang, Chunyong;Wang, Hongshui;Yang, Xianjin
种植体的表面微观结构和化学成分对其体内骨结合具有重要意义。本文采用飞秒激光（FSL）照射羟基磷灰石悬浮液中的钛（Ti）种植体表面，在其表面形成了一个由磷酸钙（Ca/P相）覆盖的分级微图案。成骨细胞的钙/磷时相和细胞层次结构增加了细胞的粘附。在2.55 J/cm（2）的FSL处理的表面上，成骨标记物（骨钙素、骨桥蛋白和侏儒相关转录因子-2）的表达更高，表明激光处理对细胞分化的有利影响。在体内研究进行了评估的影响，激光治疗和钙/磷沉积的骨整合。结果表明，经FSL处理的钛种植体与骨的结合能力明显强于抛光或喷砂酸蚀（SLA）钛种植体与骨的结合能力。植入8周后，FSL处理的植入物周围无纤维组织，骨小梁。此外，在FSL处理的种植体周围观察到更高的骨矿物质密度。我们的体外和体内研究表明，FSL诱导的微图案和Ca/P相对加速钛种植体与骨组织的早期骨结合具有积极作用。
Surface microstructure and chemical composition of the implant are very important for its osseointegration in vivo. In this paper, a hierarchical micropattern covered with calcium phosphate (Ca/P phase) was obtained on titanium (Ti) implant surface by femtosecond lasers (FSL) irradiation in hydroxyapatite suspension. The hierachical micropattern as well as Ca/P phase increased osteoblastic cell adhesion. Higher expression of osteogenic markers (osteocalcin, osteopontin, and runt related transcription factor-2) on the surface treated by FSL of 2.55 J/cm(2) indicated the favorable effect of laser treatment on cell differentiation. In vivo studies were carried out to evaluate the effect of laser treatment and Ca/P deposition on the osseointegration. It showed that the binding capacity between bone and FSL-treated Ti implants was obviously stronger than that between bone and polished or sand blasting and acid etching (SLA) Ti implants. Bone trabecula surrounded the FSL-treated implants without fibrous tissue after 8-week implantation. Also, higher bone mineral density was seen surrounding the FSL-treated implants. Our in vitro and in vivo studies demonstrated that the FSL induced micropattern and Ca/P phase had positive effects on the acceleration of early osseointegration of Ti implants with bone tissue.