Isoelectric point determination of norovirus virus-like particles by capillary Isoelectric focusing with whole column imaging detection
Isoelectric point determination of norovirus virus-like particles by capillary Isoelectric focusing with whole column imaging detection
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DOI：
10.1021/ac034848s
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发表时间：
2004-01-01
影响因子：
7.4
通讯作者：
Pawliszyn, J
中科院分区：
文献类型：
--
作者：
Goodridge, L;Goodridge, C;Pawliszyn, J
采用毛细管等电聚焦-全柱成像检测（CIEF-WCID）方法测定诺如病毒病毒样颗粒（VLP）的等电点（pI）。 VLP 由代表两个基因组的诺如病毒产生，即基因组 I（船桥、濑户和诺沃克）和基因组 11（夏威夷、柏和成田）。使用成像 CIEF-WCID 检测技术，使用短 (4-5 cm) 内涂层毛细管（直径 100 μm）和在 280 nm 下操作的全柱光学吸收成像检测器完成分离。 CIEF-WCID实验显示基因组I和基因组11的VLP的pI值相似，船桥、诺沃克、濑户和夏威夷的pI值为5.9、5.9、6.0和6.0。另外两个 VLP 显示的 pI 值为 5.5（柏）和 6.9（成田）。 VLP 峰已被证明可重现。 CIEF-WCID 在公共卫生、临床和食品安全应用中展示了诺如病毒检测的巨大前景，因为 CIEF-WCID 克服了当前使用的遗传和免疫学方法的一些局限性。
A capillary isoelectric focusing-whole column imaging detection (CIEF-WCID) method was used to determine the isoelectric point (pI) of norovirus virus-like particles (VLPs). The VLPs were produced from noroviruses that represented the two genogroups, genogroup I (Funabashi, Seto, and Norwalk) and genogroup, 11 (Hawaii, Kashiwa, and Narita). Using the imaged CIEF-WCID detection technique, separation was accomplished using a short (4-5 cm) internally coated capillary (100-mum diameter) and a whole-column optical absorption imaging detector operated at 280 nm. CIEF-WCID experiments showed the similarity of the pI values of VLPs from genogroups I and 11, with pI values of 5.9, 5.9, 6.0, and 6.0 for Funabashi, Norwalk, Seto, and Hawaii. The two other VLPs displayed pI values of 5.5 (Kashiwa) and 6.9 (Narita). The VLP peaks were shown to be reproducibility resolved. CIEF-WCID shows great promise for norovirus detection in public health, clinical, and food safety applications, as CIEF-WCID overcomes several limitations of the currently used genetic and immunological methods.