Localization of immunoreactive alpha-bag-cell peptide in the central nervous system of Aplysia.
Localization of immunoreactive alpha-bag-cell peptide in the central nervous system of Aplysia.
复制标题
海兔中枢神经系统中免疫反应性α袋细胞肽的定位。
DOI：
10.1002/cne.902870409
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Blankenship,JE
中科院分区：
文献类型：
--
作者：
Painter,SD;Kalman,VK;Nagle,GT;Blankenship,JE
海洋软体动物的袋状细胞是一种具有良好特征的神经内分泌细胞，可以启动产卵，但触发袋状细胞活动的自然刺激尚未确定。作为鉴定可能向袋细胞网络提供突触或神经激素输入的中枢神经元的第一步，产生了针对α -袋细胞肽（α - BCP）的特异性抗体。这种肽属于一个结构相关的小家族，可以在体外诱导袋细胞活性。抗体特异性通过免疫点测定和预吸收研究确定：当抗体与α - BCP -甲状腺球蛋白偶联物或α - BCP -(1-8)预孵育时，每个神经节中的免疫细胞化学标记被取消，但与甲状腺球蛋白或甲状腺球蛋白-甲状腺球蛋白偶联物预孵育不受影响。抗体特异性标记腹神经节的袋细胞和腹、右胸膜神经节的异位袋细胞。异位袋细胞在大小和形态上与常规袋细胞相似，但在数量和位置上不同。在大脑神经节中，抗体在神经节背表面标记了两侧对称的一对细胞团，每个细胞约含有10个细胞。脑细胞比袋细胞小，位置恒定，并将其过程发送到神经细胞而不是结缔组织鞘。在脑神经节、胸膜神经节和足神经节的神经粒以及脑足神经节、脑胸膜神经节和胸膜内脏连接的轴突中观察到免疫反应过程。颊神经节和足神经节未见免疫反应性细胞体。同样的标记模式被观察到在加利福尼亚的应用程序，A. Brasiliana，和。Dactylomela。因此，这三种动物在食管周围神经节内的免疫反应性细胞体分布与心房腺肽B（一种结构上与α - BCP相关的肽）体外诱导袋细胞活性的接受位点分布相似。这些观察结果表明，在这些研究中发现的免疫反应性细胞，或其中的一部分，可能参与了袋细胞活性的生理诱导。然而，由于低剂量的α - BCP对袋细胞有额外的抑制作用，因此鉴定的细胞可能在袋细胞活性的调节中发挥更复杂的作用。
The bag cells of the marine molluscAplysiaare well‐characterized neuroendocrine cells that initiate egg laying, but the natural stimulus triggering bag‐cell activity has not been determined. As a first step toward identifying central neurons that might provide synaptic or neurohormonal input onto the bag‐cell network, antibodies specific for alpha‐bag‐cell peptide (α‐BCP) were generated. This peptide belongs to a small family of structurally related peptides that can elicit bag‐cell activity in vitro. Antibody specificity was established by immunodot assay and preabsorption studies: immunocytochemical labeling was abolished in each ganglion when the antibodies were preincubated with either α‐BCP‐thyroglobulin conjugate or α‐BCP‐(1–8) but was not affected by preincubation with thyroglobulin or thyroglobulin‐thyroglobulin conjugate. The antibodies specifically labeled the bag cells in the abdominal ganglion and ectopic bag cells in both the abdominal and right pleural ganglia. The ectopic bag cells were similar to conventional bag cells in size and morphology, but varied in number and location among preparations. In the cerebral ganglion, the antibodies labeled a bilaterally symmetrical pair of cell clusters, containing approximately ten cells each, on the dorsal surface of the ganglion. The cerebral cells were smaller than bag cells, were constant in location, and sent their processes into the neuropil rather than the connective tissue sheath. Immunoreactive processes were observed in the neuropils of the cerebral, pleural, and pedal ganglia and among the axons of the cerebropedal, cerebropleural, and pleurovisceral connectives. No immunoreactive cell bodies were observed in the buccal or pedal ganglia. Identical patterns of labeling were observed inAplysia californica, A. Brasiliana, andA. Dactylomela.The distribution of immunoreactive cell bodies within the circumesophageal ganglia of all three species thus parallels the distribution of receptive sites for the in vitro induction of bag‐cell activity by atrial gland peptide B, a peptide structurally related to α‐BCP. These observations suggest that the immunoreactive cells identified in these studies, or a subset of them, may be involved in the physiological induction of bag‐cell activity. Since low doses of α‐BCP have additional inhibitory actions on the bag cells, however, it is possible that the identified cells could play a more complex role in the regulation of bag‐cell activity.