The utility of whole genome amplification for typing compromised forensic samples
The utility of whole genome amplification for typing compromised forensic samples
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DOI：
10.1111/j.1556-4029.2006.00262.x
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发表时间：
2006-11-01
影响因子：
1.6
通讯作者：
Foran, David R.
中科院分区：
文献类型：
--
作者：
Barber, Amy L.;Foran, David R.
生物证据在犯罪实验室中已变得无比珍贵；然而，其数量和/或质量可能有限。鉴于此，以无偏差的方式扩增从证据中获取的总DNA的能力将极具优势。全基因组扩增（WGA）方法有可能发挥这一作用，从而产生几乎无限的DNA供应。在本次研究中，使用商业试剂盒对两种WGA方法——改良的引物延伸预扩增和多重置换扩增（MDA）进行了测试。对照DNA、人工降解的DNA以及来自新鲜血液、陈旧血液、毛干和陈旧骨骼的DNA都进行了WGA，随后进行了短串联重复序列和线粒体DNA分析。这些方法确实扩增了DNA，但在法医相关样本上表现不佳；最大扩增子大小减小，并且MDA在聚合酶链反应后经常产生额外的条带。综上所述，除非样本质量非常高，否则WGA在法医上的应用似乎有限。
Biological evidence has become invaluable in the crime laboratory; however, it may exist in limited quantity and/or quality. Given this, the ability to amplify total DNA obtained from evidence, in an unbiased manner, would be highly advantageous. Methods for whole genome amplification (WGA) have the potential to fulfill this role, resulting in a virtually unlimited supply of DNA. In the research presented, two WGA methods, improved primer extension preamplification and multiple displacement amplification (MDA), were tested using commercial kits. Control DNA, artificially degraded DNA, and DNA from fresh blood, aged blood, hair shafts, and aged bones underwent WGA, followed by short tandem repeat and mitochondrial DNA analysis. The methods did amplify DNA, but performed poorly on forensically relevant samples; the maximum amplicon size was reduced, and MDA often resulted in extraneous bands following polymerase chain reaction. Taken together, WGA appears to be of limited forensic utility unless the samples are of a very high quality.