Identification of tissue-restricted transcripts in human islets
Identification of tissue-restricted transcripts in human islets
复制标题
DOI：
10.1210/en.2004-0691
复制
发表时间：
2004-10-01
期刊：
影响因子：
4.8
通讯作者：
Harris, PE
中科院分区：
文献类型：
--
作者：
Maffei, A;Liu, ZR;Harris, PE
我们研究的目的是确定特定的组织限制，膜相关蛋白人类胰岛，反过来，可能作为健康或患病的胰岛细胞质量的标志物的转录本。使用寡核苷酸芯片，我们获得了人类胰岛的基因表达谱，与胰腺外分泌，肝脏和肾脏组织的基因表达谱进行比较。由于胰岛周围存在1型干扰素与1型糖尿病的发展有关，因此还测定了用干扰素-α 2 β（IFN α 2 β）离体处理的人胰岛的表达谱。一组基因编码的跨膜或膜相关的蛋白质与新的胰岛限制性表达的解决，通过确定的交集的胰岛集与其他组织获得的数据集的补充。在IFN α 2 β的影响下，几种鉴定的基因产物的转录本的表达水平被上调或下调。在这项研究中确定的胰岛限制性基因产物之一，囊泡单胺转运蛋白2型，被证明结合[H-3]二氢丁苯那嗪，配体与衍生物适用于正电子发射断层扫描成像。我们在这里报告的第一个比较人类胰岛与其他组织的基因表达谱，并确定可能用于确定胰岛细胞质量的靶分子。
The purpose of our study was to identify transcripts specific for tissue-restricted, membrane-associated proteins inhuman islets that, in turn, might serve as markers of healthy or diseased islet cell masses. Using oligonucleotide chips, we obtained gene expression profiles of human islets for comparison with the profiles of exocrine pancreas, liver, and kidney tissue. As periislet presence of type 1 interferon is associated with the development of type 1 diabetes, the expression profile of human islets treated ex vivo with interferon-alpha2beta (IFNalpha2beta) was also determined. A set of genes encoding transmembrane- or membrane-associated proteins with novel islet-restricted expression was resolved by determining the intersection of the islet set with the complement of datasets obtained from other tissues. Under the influence of IFNalpha2beta, the expression levels of transcripts for several of the identified gene products were up- or down-regulated. One of the islet-restricted gene products identified in this study, vesicular monoamine transporter type 2, was shown to bind [H-3] dihydrotetrabenazine, a ligand with derivatives suitable for positron emission tomography imaging. We report here the first comparison of gene expression profiles of human islets with other tissues and the identification of a target molecule with possible use in determining islet cell masses.