Functional similarities between the dictyostelium protein AprA and the human protein dipeptidyl-peptidase IV.
Functional similarities between the dictyostelium protein AprA and the human protein dipeptidyl-peptidase IV.
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盘基网柄菌蛋白 AprA 和人蛋白二肽基肽酶 IV 之间的功能相似性。
DOI：
10.1002/pro.3107
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Gomer,RichardH
中科院分区：
文献类型：
--
作者：
Herlihy,SarahE;Tang,Yu;Phillips,JonathanE;Gomer,RichardH
自分泌增殖抑制蛋白A（Autocrine proliferation repressor protein A，AprA）是由盘基网柄藻细胞分泌的一种蛋白质。尽管AprA与任何人类蛋白质之间的序列相似性非常小，但AprA与人类蛋白质二肽基肽酶IV（DPPIV）具有预测的结构相似性。AprA对网柄细胞有化学排斥作用，DPPIV对中性粒细胞有化学排斥作用。这导致我们研究AprA和DPPIV是否具有额外的功能相似性。我们发现DPPIV和AprA一样，对D. AprA与一些DPPIV结合伙伴如纤连蛋白结合。相反，rAprA具有DPPIV样蛋白酶活性。这些结果表明，两个真核细胞的chemorepellent蛋白质的序列相似性非常小的功能相似性，并强调使用预测的蛋白质结构，以搜索蛋白质结构数据库的有用性，除了寻找具有相似序列的蛋白质。
Autocrine proliferation repressor protein A (AprA) is a protein secreted byDictyostelium discoideumcells. Although there is very little sequence similarity between AprA and any human protein, AprA has a predicted structural similarity to the human protein dipeptidyl peptidase IV (DPPIV). AprA is a chemorepellent forDictyosteliumcells, and DPPIV is a chemorepellent for neutrophils. This led us to investigate if AprA and DPPIV have additional functional similarities. We find that like AprA, DPPIV is a chemorepellent for, and inhibits the proliferation of,D. discoideumcells, and that AprA binds some DPPIV binding partners such as fibronectin. Conversely, rAprA has DPPIV‐like protease activity. These results indicate a functional similarity between two eukaryotic chemorepellent proteins with very little sequence similarity, and emphasize the usefulness of using a predicted protein structure to search a protein structure database, in addition to searching for proteins with similar sequences.