Demonstration of Cu-Zn superoxide dismutase in rat liver peroxisomes. Biochemical and immunochemical evidence.
Demonstration of Cu-Zn superoxide dismutase in rat liver peroxisomes. Biochemical and immunochemical evidence.
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DOI：
10.1016/s0021-9258(19)50508-7
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发表时间：
1992-04
期刊：
影响因子：
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通讯作者：
G. Dhaunsi;S. Gulati;Avtar K. Singhlg;J. Orak;Kohtaro Asayamall;Inderjit Singhit
中科院分区：
文献类型：
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作者：
G. Dhaunsi;S. Gulati;Avtar K. Singhlg;J. Orak;Kohtaro Asayamall;Inderjit Singhit
在本研究中，我们利用高纯度的大鼠肝脏过氧化物体，从分析细胞分离、Western印迹和免疫细胞化学分析中提供了动物过氧物体中存在铜锌超氧化物歧化酶的证据。用过氧化物酶体增殖剂环丙贝特处理后，过氧化物体超氧化物歧化酶活性增加了3倍，但对线粒体活性无影响，但胞浆超氧化物歧化酶活性显著降低，进一步支持了铜锌超氧化物歧化酶除胞质和线粒体定位外，还存在于过氧化体中。超氧化物歧化酶在过氧化物体中的表现表明，这种细胞器在缺血再灌注损伤等病理生理条件下具有新的作用。
In this study, by using highly purified rat liver peroxisomes, we provide evidence from analytical cell fractionation, Western blot, and immunocytochemical analysis that Cu-Zn superoxide dismutase is present in animal peroxisomes. Treatment with ciprofibrate, a peroxisome proliferator, increased the peroxisomal superoxide dismutase activity by 3-fold with no effect on mitochondrial activity but a marked decrease in cytosolic superoxide dismutase activity, further supporting that besides cytosolic and mitochondrial localization, Cu-Zn superoxide dismutase is present in peroxisomes also. Demonstration of superoxide dismutase in peroxisomes suggests a new role for this organelle in pathophysiological conditions, such as ischemia-reperfusion injury.