Characterization of protein-protein interactions between rice viruses and vector insects
Characterization of protein-protein interactions between rice viruses and vector insects
复制标题
水稻病毒与媒介昆虫之间蛋白质-蛋白质相互作用的表征
DOI：
10.1111/1744-7917.12840
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发表时间：
2020
期刊：
影响因子：
4
通讯作者：
Cui Feng
中科院分区：
文献类型：
--
作者：
Zhu Junjie;Eid Fatma Elzahraa;Tong Lu;Zhao Wan;Wang Wei;Heath Lenwood S.;Kang Le;Cui Feng
稻飞虱是最臭名昭著的水稻害虫，因为它们以持续繁殖的方式传播各种水稻病毒。病毒与媒介之间的蛋白质相互作用（PPI）是病毒通过媒介昆虫传播的关键。然而，已知的水稻病毒和稻飞虱对的PPI的数量受到低通量研究方法的限制。在这项研究中，我们应用DeNovo，一种基于病毒宿主序列的PPI预测因子，在全基因组范围内预测三种稻飞虱和五种水稻病毒之间的潜在PPI。PPI在两个不同的置信阈值下被识别，称为低和高模式。在低水平模式下，5种稻飞虱病毒对的PPI数量范围为506至1985，在高水平模式下，PPI数量范围为1254至4286。在消除“一个太多”的冗余相互作用信息后，具有独特稻飞虱蛋白的PPI在低模式下减少到343-724，在高模式下减少到758-1671。同源性分析表明，两种模式下的所有稻飞虱-病毒互作共有11套和31套稻飞虱同源蛋白，表明它们可能是水稻病毒传播所必需的保守载体因子。利用谷胱甘肽-S-转移酶（GST）/His-pull down或免疫共沉淀法，对10种褐飞虱与水稻条纹病毒（RSV）的PPI进行了验证。10种PPI中有5种被证实为阳性，5种SBPH蛋白中有3种被证实与RSV相互作用。预测的PPIs为进一步研究水稻病毒与其媒介昆虫之间的复杂关系提供了新的线索。
Planthoppers are the most notorious rice pests, because they transmit various rice viruses in a persistent‐propagative manner. Protein–protein interactions (PPIs) between virus and vector are crucial for virus transmission by vector insects. However, the number of known PPIs for pairs of rice viruses and planthoppers is restricted by low throughput research methods. In this study, we applied DeNovo, a virus‐host sequence‐based PPI predictor, to predict potential PPIs at a genome‐wide scale between three planthoppers and five rice viruses. PPIs were identified at two different confidence thresholds, referred to as low and high modes. The number of PPIs for the five planthopper‐virus pairs ranged from 506 to 1985 in the low mode and from 1254 to 4286 in the high mode. After eliminating the “one‐too‐many” redundant interacting information, the PPIs with unique planthopper proteins were reduced to 343–724 in the low mode and 758–1671 in the high mode. Homologous analysis showed that 11 sets and 31 sets of homologous planthopper proteins were shared by all planthopper‐virus interactions in the two modes, indicating that they are potential conserved vector factors essential for transmission of rice viruses. Ten PPIs between small brown planthopper and rice stripe virus (RSV) were verified using glutathione‐S‐transferase (GST)/His‐pull down or co‐immunoprecipitation assay. Five of the ten PPIs were proven positive, and three of the five SBPH proteins were confirmed to interact with RSV. The predicted PPIs provide new clues for further studies of the complicated relationship between rice viruses and their vector insects.