Andalucia (n. Gen.) -: the deepest branch within jakobids (Jakobida;: Excavata), based on morphological and molecular study of a new flagellate from soil
Andalucia (n. Gen.) -: the deepest branch within jakobids (Jakobida;: Excavata), based on morphological and molecular study of a new flagellate from soil
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DOI：
10.1111/j.1550-7408.2005.00081.x
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发表时间：
2006-03-01
影响因子：
2.2
通讯作者：
Simpson, AGB
中科院分区：
文献类型：
--
作者：
Lara, E;Chatzinotas, A;Simpson, AGB
本文报道了一种新的异养鞭毛虫（Andalucia godoyi n.一般的;一般的种）从土壤中描述。早期的初步18 S rRNA分析表明，与遗传学上难以放置的jakobid Jakoba incorporata有关系。安达卢西亚godoyi是一个小的（3-5 μ m）双鞭毛细胞与腹面的饲料槽。它有管状线粒体嵴。有两个主要的微管根（R1，R2）和一个与基体1（后部）相关的单线根。微管根R1与非微管纤维“I”、“B”和“A”相关联，并分成两部分，而R2与“C”纤维相关联。这些结构支撑沟的前部。A. godoyi是jakobids的特征：（i）在鞭毛2上有一个单一的背叶片;（ii）C纤维具有jakobid多层亚结构;（iii）微管的背扇起源于与基体2非常密切的联系;（iv）没有与基体2相关的“R4”微管根。形态学分析结合A. godoyi数据强烈支持所有jakobids的单系性。我们的18 S rRNA基因将A. godoyi和J. incorporata 1个强分支，与其它亚科的分支福尔斯分开。统计检验并不否定jakobid单系性，但Jakoba libera与J. incorporata和/或A. godoyi被拒绝了。因此，我们建立了一个新属--安达卢西亚属Andalucia n.模式种Andalucia godoyi n. sp.，并将Jakoba incarcinata转移到Andalucia作为Andalucia incarcinata n. comb.
A new heterotrophic flagellate (Andalucia godoyi n. gen. n. sp.) is described from soil. Earlier preliminary 18S rRNA analyses had indicated a relationship with the phylogenetically difficult-to-place jakobid Jakoba incarcerata. Andalucia godoyi is a small (3-5 mu m) biflagellated cell with a ventral feeding groove. It has tubular mitochondrial cristae. There are two major microtubular roots (R1, R2) and a singlet root associated with basal body 1 (posterior). The microtubular root R1 is associated with non-microtubular fibres "I," "B," and "A," and divides in two parts, while R2 is associated with a "C" fibre. These structures support the anterior portion of the groove. Several features of A. godoyi are characteristic of jakobids: (i) there is a single dorsal vane on flagellum 2; (ii) the C fibre has the jakobid multilaminate substructure; (iii) the dorsal fan of microtubules originates in very close association with basal body 2; and (iv) there is no "R4" microtubular root associated with basal body 2. Morphological analyses incorporating the A. godoyi data strongly support the monophyly of all jakobids. Our 18S rRNA phylogenies place A. godoyi and J. incarcerata as a strong clade, which falls separately from other jakobids. Statistical tests do not reject jakobid monophyly, but a specific relationship between Jakoba libera and J. incarcerata and/or A. godoyi is rejected. Therefore, we have established a new genus Andalucia n. gen. with the type species Andalucia godoyi n. sp., and transfer Jakoba incarcerata to Andalucia as Andalucia incarcerata n. comb.