Longterm stability and developmental changes in spontaneous network burst firing patterns in dissociated rat cerebral cortex cell cultures on multielectrode arrays
Longterm stability and developmental changes in spontaneous network burst firing patterns in dissociated rat cerebral cortex cell cultures on multielectrode arrays
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DOI：
10.1016/j.neulet.2003.12.062
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发表时间：
2004-05-06
影响因子：
2.5
通讯作者：
Ramakers, GJA
中科院分区：
文献类型：
--
作者：
Van Pelt, J;Corner, MA;Ramakers, GJA
将分离的大鼠枕皮质细胞在平面多电极板上培养4-7周，纵向记录其从孤立神经元向突触连接神经元网络发育过程中的自发动作电位。活动通常由持续数秒的广泛爆发组成，在一些活动地点由不同时期的零星射击分开。这些网络爆发的放电模式与年龄有关，持续时间在体外培养第3周显著增加，在体外培养约1个月后逐渐减少，并演变为短时间的突发事件。这些发现表明，在体外培养大约一个月后，这些培养的神经网络已经发展出一定程度的兴奋性，几乎可以瞬时触发全身性放电。在这些网络爆发中，单个神经元倾向于以特定和持久的时间关系彼此放电，这表明网络连接在其发展过程中保持了核心拓扑结构。2003爱思唯尔爱尔兰有限公司版权所有。
Spontaneous action potentials were recorded longitudinally for 4-7 weeks from dissociated rat occipital cortex cells cultured on planar multi-electrode plates, during their development from isolated neurons into synaptically connected neuronal networks. Activity typically consisted of generalized bursts lasting up to several seconds, separated by variable epochs of sporadic firing at some of the active sites. These network bursts displayed discharge patterns with age-dependent firing rate profiles, and durations significantly increasing in the 3rd week in vitro and decreasing after about I month in vitro, when they evolved into short events with prompt onsets. These findings indicate that after about a month in vitro these cultured neuronal networks have developed a degree of excitability that allows almost instantaneous triggering of generalized discharges. Individual neurons tend to fire in specific and persistent temporal relationships to one another within these network bursts, suggesting that network connectivity maintains a core topology during its development. (C) 2003 Elsevier Ireland Ltd. All rights reserved.