Effects of acute or chronic environmental enrichment on regional Fos protein expression following sucrose cue-reactivity testing in rats.
Effects of acute or chronic environmental enrichment on regional Fos protein expression following sucrose cue-reactivity testing in rats.
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DOI：
10.1007/s00429-015-1074-z
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发表时间：
2016-06
影响因子：
3.1
通讯作者：
Eaton L
中科院分区：
文献类型：
--
作者：
Grimm JW;Barnes JL;Koerber J;Glueck E;Ginder D;Hyde J;Eaton L
暴露于环境富集（EE）减少了蔗糖自我给药历史的大鼠寻求蔗糖。本实验研究了急性或慢性EE是否也会降低脑Fos水平，这是一种指示神经元激活的蛋白质标志物。在强迫禁食1天或30天后也检测了Fos水平，以检查Fos水平是否随蔗糖渴望的潜伏期而变化。在18个脑区检测Fos的表达，并用免疫组化方法在脑切片中鉴定Fos的表达。强迫禁欲30天后，大多数地区的Fos水平较高，急性或慢性EE在大多数地区都有所下降。11个地区对Fos有统计学上显著的影响和/或相互作用；这里列出了其中最突出的几点。在前边缘皮层中，在两个强制戒断时间点都有Fos和EE的孵育。相比之下，在眶额皮质没有Fos孵育，但在两个强制禁欲时间点，EE降低了Fos。在Fos孵育的前扣带皮层和伏隔核核壳中观察到EE和孵育的相互作用，但EE仅在第30天强制戒断时间点降低Fos。相反，在背外侧纹状体中，Fos孵育，但EE在两个强制禁欲时间点都显著降低了Fos的表达。这些差异表达模式为更详细的、特定位点的分子功能研究提供了理论依据，研究它们与EE减少蔗糖寻找能力的关系。
Exposure to environmental enrichment (EE) reduces sucrose seeking by rats with a history of sucrose self-administration. The present experiment examined whether acute or chronic EE also reduces brain Fos levels, a protein marker indicative of neuronal activation. Fos levels were also examined after either 1 or 30 days of forced abstinence to examine whether Fos levels vary with the incubation of sucrose craving. Fos expression was examined in 18 regions and was identified in brain slices using immunohistochemistry. Fos levels were higher in most regions after 30 d of forced abstinence and were decreased in most regions by either acute or chronic EE. Eleven regions had some statistically significant effect and/or interaction of EE or incubation on Fos; the most salient of these are listed here. In the prelimbic cortex there was an incubation of Fos and EE reduced Fos at both forced abstinence time points. In contrast, in the orbitofrontal cortex there was no Fos incubation but EE reduced Fos at both forced abstinence time points. An interaction of EE and incubation was observed in the anterior cingulate cortex and nucleus accumbens core and shell where Fos incubated but EE only decreased Fos at the Day 30 forced abstinence time point. In contrast, in the dorsolateral striatum Fos incubated, but EE robustly decreased Fos expression at both forced abstinence time points. These differential expression patterns provide rationale for more detailed, site-specific molecular functional studies in how they relate to the ability of EE to reduce sucrose seeking.