Outbreak of betanodavirus infection in tilapia, Oreochromis niloticus (L.), in fresh water
Outbreak of betanodavirus infection in tilapia, Oreochromis niloticus (L.), in fresh water
复制标题
DOI：
10.1111/j.1365-2761.2009.01037.x
复制
发表时间：
2009-08-01
影响因子：
2.5
通讯作者：
Castric, J.
中科院分区：
文献类型：
--
作者：
Bigarre, L.;Cabon, J.;Castric, J.
从30 ℃淡水中饲养的罗非鱼Oreochromis niloticus的幼虫中分离出了一种与大量死亡率相关的β-结节病毒。组织学检查显示神经系统空泡化，提示β-结节病毒感染。在SSN 1细胞系中通过间接荧光抗体试验鉴定病毒，并通过PCR产物测序进一步表征。外壳蛋白基因T4区的测序表明，该分离物的系统发育聚类内的红斑石斑鱼神经坏死病毒类型。然而，罗非鱼分离株形成了与其他贝塔诺病毒分离株不同的独特分支。通过在30摄氏度下对幼罗非鱼进行水浴感染，实验性地复制了这种疾病。爆发的病毒宿主仍未查明。据我们所知，这是第一次在淡水养殖的罗非鱼中自然感染诺达病毒的报道。
A betanodavirus associated with a massive mortality was isolated from larvae of tilapia, Oreochromis niloticus, maintained in fresh water at 30 degrees C. Histopathology revealed vacuolation of the nervous system, suggesting an infection by a betanodavirus. The virus was identified by indirect fluorescent antibody test in the SSN1 cell line and further characterized by sequencing of a PCR product. Sequencing of the T4 region of the coat protein gene indicated a phylogenetic clustering of this isolate within the red-spotted grouper nervous necrosis virus type. However, the tilapia isolate formed a unique branch distinct from other betanodavirus isolates. The disease was experimentally reproduced by bath infection of young tilapia at 30 degrees C. The reservoir of virus at the origin of the outbreak remains unidentified. To our knowledge, this is the first report of natural nodavirus infection in tilapia reared in fresh water.