INTERLEUKIN-8 AND THE NEUTROPHIL RESPONSE TO MUCOSAL GRAM-NEGATIVE INFECTION
INTERLEUKIN-8 AND THE NEUTROPHIL RESPONSE TO MUCOSAL GRAM-NEGATIVE INFECTION
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DOI：
10.1172/jci116650
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发表时间：
1993-08-01
影响因子：
15.9
通讯作者：
SVANBORG, C
中科院分区：
文献类型：
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作者：
AGACE, WW;HEDGES, SR;SVANBORG, C
尿路感染激活粘膜炎症反应，包括细胞因子分泌和中性粒细胞内流。中性粒细胞内流的机制尚未确定。白细胞介素-8是一种有效的中性粒细胞化学引诱剂，在体外由尿路上皮细胞系产生。本研究分析了人IL-8对故意感染尿路大肠杆菌的反应。膀胱内灌注大肠杆菌前后分别取尿液和血清样本。测定未离心尿液中中性粒细胞数量，ELISA法测定白细胞介素-8水平。细菌滴注后所有患者尿IL-8均有反应，但血清IL-8未检测到。尿IL-8水平与尿中性粒细胞数量有很强的相关性。用于定植患者的大肠杆菌菌株刺激尿路上皮细胞中IL-8的产生。上皮细胞系分泌的IL-8水平受大肠杆菌菌毛特性的影响。这些结果表明，大肠杆菌引起人粘膜IL-8反应，并提示IL-8参与脓尿的发病。上皮细胞可能是尿路感染过程中IL-8的重要来源。
Urinary tract infections activate a mucosal inflammatory response, which includes cytokine secretion and neutrophil influx. The mechanisms involved in the neutrophil influx have not been identified. Interleukin-8, a potent chemoattractant for neutrophils, is produced by urinary tract epithelial cell lines in vitro. This study analyzed the human IL-8 response to deliberate Escherichia coli infection of the urinary tract. Urine and serum samples were obtained before and after intravesical instillation of E. coli. Neutrophil numbers were determined on uncentrifuged urine, and IL-8 levels were measured by ELISA. A urinary IL-8 response was found in all patients after bacterial instillation, but no serum IL-8 was detected. There was a strong correlation between urinary IL-8 levels and urinary neutrophil numbers. The same E. coli strains used to colonize the patients stimulated IL-8 production in urinary tract epithelial cells. The level of IL-8 secreted by epithelial cell lines was influenced by the fimbrial properties of the E. coli. These results demonstrated that E. coli elicit a mucosal IL-8 response in humans, and suggested that IL-8 is involved in the onset of pyuria. Epithelial cells may be an important source of IL-8 during urinary tract infection.