Expression profiles of two novel lipoxygenase genes in Populus deltoides
Expression profiles of two novel lipoxygenase genes in Populus deltoides
复制标题
DOI：
10.1016/j.plantsci.2005.12.012
复制
发表时间：
2006-06-01
期刊：
影响因子：
5.2
通讯作者：
Huang, Minren
中科院分区：
文献类型：
--
作者：
Cheng, Qiang;Zhang, Bo;Huang, Minren
本研究从美洲黑杨中克隆了两个脂氧合酶基因PdLOX 1和PdLOX 2（GenBank登录号：DQ 131178，DQ 131179）。“勒克斯”（1-69/55）。PdLOX 1和PdLOX 2的原核表达分析表明，其编码的外源蛋白与预期的分子量一致，并具有预期的脂氧合酶活性。色谱分析表明，这两种脂氧合酶主要具有13-LOX活性。已知脂氧合酶的衍生氨基酸序列的系统发育分析显示，PdLOX 1和PdLOX 2是2型13-LOX基因家族的成员。已知这类脂氧合酶参与生物和非生物胁迫。使用实时RT-PCR，我们评估了暴露于白杨真菌病原体（褐斑盘菌f. Mieltigermtubi）、机械损伤、茉莉酸甲酯（MeJA）或水杨酸（SA）。我们报告，PdLOX 1和PdLOX 2的表达水平增加后，暴露于M。布伦内亚湾sp. Multigermtubi，其中病原菌对PdLOX 1表达的影响相对较强。此外，这两个基因的表达水平也上调机械损伤和暴露于MeJA。与此相反，PdLOX 1和PdLOX 2的表达下调SA处理。推测这两种新的脂氧合酶可能在白杨抗生物和非生物胁迫中发挥重要作用。(c)2006爱思唯尔爱尔兰有限公司保留所有权利。
In the present study, we cloned two lipoxygenase genes, PdLOX1 and PdLOX2 (GenBank accession no. DQ131178,DQ131179), from Populus deltoides cv. 'Lux' (1-69/55). A prokaryotic expression analysis of PdLOX1 and PdLOX2 revealed that the encoded exogenous proteins were identical to the predicted molecular weights and possessed the expected lipoxygenase activities. Chromatogram analysis indicated that the two lipoxygenase mainly possess 13-LOX activity. Phylogenetic analysis of the derived amino acid sequences of known lipoxygenases revealed that PdLOX1 and PdLOX2 were members of the type 2 13-LOX family of genes. This class of lipoxygenases is known to be involved in biotic and abiotic stress. Using real-time RT-PCR, we evaluated PdLOX1 and PdLOX2 expression following exposure to a Poplar fungal pathogen (Marssonina brunnea f. sp. Mieltigermtubi), mechanical injury, methyl jasmonate (MeJA), or salicylic acid (SA). We report that both PdLOX1 and PdLOX2 expression levels were increased following exposure to M. brunnea f. sp. Multigermtubi, with the pathogen exerting a relatively stronger influence on PdLOX1 expression. Furthermore, expression levels of the two genes were also up-regulated by mechanical damage and exposure to MeJA. In contrast, both PdLOX1 and PdLOX2 expression was down-regulated by SA treatment. We propose that the two novel lipoxygenases may play an important role in Poplar resistance to biotic and abiotic stress. (c) 2006 Elsevier Ireland Ltd. All rights reserved.