Inverse regulation of Vibrio cholerae biofilm dispersal by polyamine signals.
Inverse regulation of Vibrio cholerae biofilm dispersal by polyamine signals.
复制标题
多胺信号对霍乱弧菌生物膜扩散的逆调控。
DOI：
10.7554/elife.65487
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发表时间：
2021-04-15
期刊：
影响因子：
7.7
通讯作者：
Bassler BL
中科院分区：
文献类型：
--
作者：
Bridges AA;Bassler BL
全球病原体霍乱弧菌在环境和人类宿主中经历生物膜形成和扩散的循环。对生物膜的扩散知之甚少。在这里，我们表明，MbaA，周质多胺传感器，和PotD1，多胺进口商，调节霍乱弧菌生物膜扩散。亚精胺，一种常见的多胺，驱动霍乱弧菌的传播，而去甲亚精胺，一种不常见的多胺产生的弧菌，抑制传播。亚精胺和去甲亚精胺相差一个亚甲基。这两种多胺控制分散通过MbaA检测在周质和随后的信号中继。我们的研究结果表明，分散失败的情况下，PotD1，因为内源性产生的norspermidine不重新进口，周质norspermidine积累，它刺激MbaA信号。这些结果表明，霍乱弧菌使用MbaA来监测环境多胺，其混合物可能提供有关“自我”和“他人”数量的信息。该信息用于指示是否从生物膜中分散。
The global pathogen Vibrio cholerae undergoes cycles of biofilm formation and dispersal in the environment and the human host. Little is understood about biofilm dispersal. Here, we show that MbaA, a periplasmic polyamine sensor, and PotD1, a polyamine importer, regulate V. cholerae biofilm dispersal. Spermidine, a commonly produced polyamine, drives V. cholerae dispersal, whereas norspermidine, an uncommon polyamine produced by vibrios, inhibits dispersal. Spermidine and norspermidine differ by one methylene group. Both polyamines control dispersal via MbaA detection in the periplasm and subsequent signal relay. Our results suggest that dispersal fails in the absence of PotD1 because endogenously produced norspermidine is not reimported, periplasmic norspermidine accumulates, and it stimulates MbaA signaling. These results suggest that V. cholerae uses MbaA to monitor environmental polyamines, blends of which potentially provide information about numbers of ‘self’ and ‘other’. This information is used to dictate whether or not to disperse from biofilms.