Role of the toxR Gene from Fish Pathogen Vibiro alginolyticus in the Physiology and Virulence
Role of the toxR Gene from Fish Pathogen Vibiro alginolyticus in the Physiology and Virulence
复制标题
鱼类病原菌溶藻弧菌 toxR 基因在生理学和毒力中的作用
DOI：
10.1007/s12088-017-0685-x
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发表时间：
2017-12-01
影响因子：
3
通讯作者：
Jian, Jichan
中科院分区：
文献类型：
--
作者：
Cai, Shuanghu;Cheng, Haiyan;Jian, Jichan
构建了一株框架内缺失toxR基因的溶藻弧菌突变株，以揭示toxR基因在溶藻弧菌生理和毒力中的作用。统计分析表明，野生型与突变体在生长能力、群体运动能力、胞外蛋白酶活性和注射毒力（LD50值）方面均无显著差异。然而，toxR基因的缺失会降低生物膜的形成水平。比较蛋白质组学分析表明，toxR缺失突变可上调谷氨酰胺合成酶和左旋蔗糖酶的表达，下调OmpU、DnaK等10种蛋白的表达。这些结果表明，ToxR可能通过调节谷氨酰胺合成酶、左旋蔗糖酶和OmpU的表达，增强溶藻弧菌的生物膜信息，从而影响细菌在肠道表面的定植，从而参与了感染的早期阶段。
A mutant strain of Vibiro alginolyticus with an in-frame deletion of the toxR gene was constructed to reveal the role of ToxR in the physiology and virulence of V. alginolyticus. The statistical analysis showed no significant difference in the growth ability, swarming motility, activity of extracellular protease and the virulence by injection (the value of LD50) between the wild-type and the toxR mutant. However, the deletion of toxR could decrease the level of biofilm formation. The comparative proteomic analysis demonstrated the deletion mutation of toxR could up-regulate the expression of glutamine synthetase and levansucrase, and down-regulate the expression of 10 proteins such as OmpU, DnaK, etc. These results suggest that ToxR may be involved in the early stages of infection by influencing colonization of the bacteria on the surface of the intestine through enhancing the biofilm information of V. alginolyticus via modulating the expression of glutamine synthetize, levansucrase and OmpU.