IFN-gamma priming of chicken heterophils upregulates the expression of proinflammatory and Th1 cytokine mRNA following receptor-mediated phagocytosis of Salmonella enterica serovar enteritidis.
IFN-gamma priming of chicken heterophils upregulates the expression of proinflammatory and Th1 cytokine mRNA following receptor-mediated phagocytosis of Salmonella enterica serovar enteritidis.
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发表时间：
2005
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通讯作者：
M. Kogut;L. Rothwell;P. Kaiser
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作者：
M. Kogut;L. Rothwell;P. Kaiser
对侵入性病原体的反应、通过炎症反应的清除以及适当的获得性反应的激活都由先天宿主防御协调。多形性白细胞（PMN）是先天性反应的细胞成分，家禽中的原发性PMN是嗜异性的。引发是吞噬细胞活化过程的增强。干扰素-γ（IFN-γ）是一种多效性细胞因子，参与免疫和炎症反应的基本所有阶段，已显示其引发嗜异性细胞功能活性。在本实验中，我们使用实时定量RT-PCR，评估重组鸡IFN-γ的作用，（rChIFN-γ）作为引发介质在促炎性细胞因子的基因转录和mRNA表达水平上控制嗜异性反应。（白细胞介素-1 β [IL-1 β]、IL-6、IL-8）和Th 1（IL-18和IFN-γ）细胞因子基因，在用吞噬作用激动剂、调理和非调理的沙门氏菌刺激后。rChIFN-γ引发异嗜性细胞增加吞噬激动剂诱导的促炎细胞因子的转录，但也上调Th 1细胞因子（IL-18和IFN-γ）mRNA的表达，并刺激IFN-γ的产生增加。尽管rChIFN-γ引发调节了不同吞噬激动剂刺激的异嗜性粒细胞中细胞因子mRNA的表达，但rChIFN-γ本身并不直接诱导促炎性或Th 1细胞因子的基因表达。细胞因子mRNA的增强表达似乎并不取决于吞噬过程中激活的受体而差异表达。本实验的结果表明，rChIFN-γ可能在禽先天免疫中对沙门氏菌感染发挥重要作用，并可能在预防和治疗新孵化的鸡沙门氏菌感染中提供辅助用途。
Responsiveness to invasive pathogens, clearance via the inflammatory response, and activation of appropriate acquired responses are all coordinated by innate host defenses. Polymorphonuclear leukocytes (PMNs) are cellular components of innate response, with the primary PMN in poultry being the heterophil. Priming is the potentiation of the phagocyte activation process. Interferon-gamma (IFN-gamma) is a pleiotropic cytokine involved in basically all phases of immune and inflammatory responses that has been shown to prime heterophil functional activities. In the present experiments, using real-time quantitative RT-PCR, we evaluated the role of recombinant chicken IFN-gamma (rChIFN-gamma) as a priming mediator to control heterophil responses at the level of gene transcription and expression of the mRNA for proinflammatory (interleukin-1beta [IL-1beta], IL-6, IL-8) and Th1 (IL-18 and IFN-gamma) cytokine genes following stimulation with phagocytosis agonists, opsonized and nonopsonized Salmonella enteritidis. rChIFN-gamma primed the heterophils for an increase in transcription of proinflammatory cytokines induced by phagocytic agonists but also upregulated expression of Th1 cytokine (IL-18 and IFN-gamma) mRNA and stimulated an increased production of IFN-gamma. Although rChIFN-gamma priming modulated the expression of cytokine mRNA in heterophils stimulated by different phagocytic agonists, rChIFN-gamma by itself did not directly induce gene expression of either proinflammatory or Th1 cytokines. The enhanced expression of cytokine mRNA does not appear to be differentially expressed depending on the receptor activated during phagocytosis. The results from the present experiments suggest that rChIFN-gamma may play a significant role in avian innate immunity against Salmonella infection and may offer an adjunct use in the prevention and treatment of salmonellae infections in newly hatched chickens.