TRYPTOPHAN TRANSFER RNA AS UGA SUPPRESSOR
TRYPTOPHAN TRANSFER RNA AS UGA SUPPRESSOR
复制标题
DOI：
10.1016/0022-2836(71)90362-7
复制
发表时间：
1971-01-01
影响因子：
5.6
通讯作者：
HIRSH
中科院分区：
文献类型：
--
作者：
HIRSH
遗传数据表明色氨酸是在大肠埃希菌CAJ64抑制UGA无义密码子的过程中插入的。来自CAJ64和相关野生型菌株CA244的色氨酸转移RNA的核苷酸序列除了二氢尿嘧啶环茎中的位置24之外是相同的。 CA244 tRNATrphas G 在该位置； CAJ64 tRNATrphas A。反密码子是 CCA，预计可单独识别色氨酸密码子 UGG。来自几种相关菌株的色氨酸 tRNA 在分离后变性，而来自抑制菌株的色氨酸 tRNA 则稳定。独立分离的 UGA 抑制菌株的 tRNATrp 与 CAJ64 相同。结论是UGA抑制是由tRNATrp进行的，tRNATrp的反密码子-密码子识别因分子中其他地方的变化而改变。
Genetic data indicate that tryptophan is inserted during suppression of the UGA nonsense codon by theEscherichia colistrain, CAJ64. The nucleotide sequences of tryptophan transfer RNA's from CAJ64 and the related wild-type strain, CA244, are identical except at position 24 in the stem of the dihydrouracil loop. CA244 tRNATrphas G at this position; CAJ64 tRNATrphas A. The anticodons are CCA, expected for recognition of the tryptophan codon, UGG, alone. Tryptophan tRNA from several relatedsu−strains denatures upon isolation, whereas that from suppressor strains is stable. An independently isolated UGA suppressor strain has tRNATrpidentical to CAJ64. It is concluded that UGA suppression is carried out by a tRNATrpwhose anticodon-codon recognition is altered by a change elsewhere in the molecule.