Liposome Solubilization Induced by Surfactant Molecules in a Microchip
Liposome Solubilization Induced by Surfactant Molecules in a Microchip
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DOI：
10.2116/analsci.28.339
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发表时间：
2012-04-01
影响因子：
1.6
通讯作者：
Katayama, Kenji
中科院分区：
文献类型：
--
作者：
Shoji, Yuri;Igarashi, Takashi;Katayama, Kenji
通过动态光散射和光学显微镜监测脂质体溶解的动态。将新设计的与房间相连的Y形微通道集成到微芯片中，将两种流体混合并停止流动后，在房间内观察脂质体悬浮液和表面活性剂溶液的反应过程。通过使用该微芯片，我们成功地实时监测了脂质体溶解情况，并提出了以下溶解的动态过程：1）变形的脂质体变成球形。 2) 脂质体尺寸增大，直到脂质体膜中的表面活性剂/脂质体比率达到阈值。 3) 表面活性剂和磷脂的混合胶束被释放，脂质体崩解。
The dynamics of liposome solubilization was monitored by dynamic light scattering and optical microscopy. A newly designed Y-shape microchannel connected to a room was incorporated into a microchip and the reaction processes of the liposome suspension and surfactant solution were observed in the room after mixing the two fluids and stopping the flow. By using this microchip, we succeeded in real-time monitoring of liposome solubilization and the following dynamic processes of solubilization were proposed: 1) Deformed liposomes become spherical. 2) The liposome size increases until the surfactant/liposome ratio in the liposome membrane reaches a threshold value. 3) Mixed micelles of surfactants and phospholipids are released and the liposomes collapse.