A Transcriptomic Analysis of Xylan Mutants Does Not Support the Existence of A Secondary Cell Wall Integrity System in Arabidopsis
A Transcriptomic Analysis of Xylan Mutants Does Not Support the Existence of A Secondary Cell Wall Integrity System in Arabidopsis
复制标题
木聚糖突变体的转录组分析不支持拟南芥中存在次生细胞壁完整性系统
DOI：
10.1101/246330
复制
发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Blanc N
中科院分区：
文献类型：
--
作者：
Blanc N
长期以来，酵母一直被认为具有细胞壁完整性（CWI）系统，最近已经描述了用于植物初生壁（PCWI）的类似系统，其导致植物生长和细胞壁组成的变化。一个类似的系统已被提出存在次生细胞壁（SCWI）。然而，几乎没有数据支持这一点。在这里，我们分析了一组细胞壁生物合成突变体的干转录组，以调查是否细胞壁损伤，在这种情况下引起的异常木聚糖合成，激活信号级联或细胞壁合成基因表达的变化。我们的数据显示转录组的变化非常少。我们假设这是因为经历次生细胞壁增厚的细胞已经进入了导致细胞死亡的承诺程序，因此SCWI系统的影响有限。对次生细胞壁改变的转录组学反应的缺乏可以促进植物次生细胞壁的工程化。
Yeast have long been known to possess a cell wall integrity (CWI) system, and recently an analogous system has been described for the primary walls of plants (PCWI) that leads to changes in plant growth and cell wall composition. A similar system has been proposed to exist for secondary cell walls (SCWI). However, there is little data to support this. Here, we analyzed the stem transcriptome of a set of cell wall biosynthetic mutants in order to investigate whether cell wall damage, in this case caused by aberrant xylan synthesis, activates a signaling cascade or changes in cell wall synthesis gene expression. Our data revealed remarkably few changes to the transcriptome. We hypothesize that this is because cells undergoing secondary cell wall thickening have entered a committed programme leading to cell death, and therefore a SCWI system would have limited impact. The absence of transcriptomic responses to secondary cell wall alterations may facilitate engineering of the secondary cell wall of plants.