A functional identification of cardiac junctional sarcoplasmic reticulum.
A functional identification of cardiac junctional sarcoplasmic reticulum.
复制标题
心脏连接肌浆网的功能鉴定。
DOI：
10.1016/0006-291x(85)90109-3
复制
发表时间：
1985
影响因子：
3.1
通讯作者：
Lattanzio,FA
中科院分区：
文献类型：
--
作者：
Brandt,NR;Brunschwig,JP;Lattanzio,FA
通过在犬心室肌微粒体中发现 calsequestrin 和 ryanodine 敏感的 Ca2+ 释放通道，可以鉴定出连接肌浆网 (SR)。然而，这些特性并不是所有物种的心肌细胞所共有的。 Seiler 等人 (1) 最近描述了犬交界 SR 中的高 Mr 多肽，类似于骨骼肌三联体的跨越蛋白亚基。我们现在报道了来自兔心室肌的 SR 中存在具有相同迁移率的多肽，并表明这些心脏膜可以与来自二甲胂酸 K 培养基中的兔骨骼肌的横（T-）小管结合。我们建议将此多肽和与 T 管的反应视为识别心脏连接 SR 的标准。
Junctional sarcoplasmic reticulum (SR) has been identified in microsomes from canine ventricular muscle by the presence of calsequestrin and ryanodinesensitive Ca2+release channels. These properties, however, are not common to cardiac cells from all species. Seiler et al (1) have recently described a high Mrpolypeptide in canine junctional SR similar to the spanning protein subunits of skeletal muscle triads. We now report the existence of a polypeptide with the same mobility in SR from rabbit ventricular muscle and show that those cardiac membranes can associate with transverse (T−) tubules from rabbit skeletal muscle in K cacodylate medium. We propose that this polypeptide and the reaction with T-tubules be considered as criteria for the identification of cardiac junctional SR.