Diverse endophytic nitrogen-fixing bacteria isolated from wild rice Oryza rufipogon and description of Phytobacter diazotrophicus gen. nov sp nov.
Diverse endophytic nitrogen-fixing bacteria isolated from wild rice Oryza rufipogon and description of Phytobacter diazotrophicus gen. nov sp nov.
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从野生稻中分离的多种内生固氮细菌和固氮植物杆菌属的描述。
DOI：
10.1007/s00203-007-0333-7
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发表时间：
2008-05-01
影响因子：
2.8
通讯作者：
Tan, Zhi Yuan
中科院分区：
文献类型：
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作者：
Zhang, Guo Xia;Peng, Gui Xiang;Tan, Zhi Yuan
从普通野生稻（Oryza rufipogon）表面灭菌的茎和根中分离到23株固氮细菌。通过SDS-PAGE蛋白质图谱将这些细菌分为4个簇，并通过IS-PCR指纹图谱分析进一步证实。16 S rRNA基因序列的系统发育分析表明，聚类II的代表菌株LS 8和LS 18形成了一个单系群，与沙门氏菌属、柠檬酸杆菌属、泛菌属、克雷伯氏菌属和肠杆菌属内的已定义的肠杆菌种具有94.0-97.3%的相似性。DNA-DNA杂交、生理生化试验和细胞形态学也表明这些菌株可以与相关的肠杆菌种区分开来。基于这些结果，我们提出重氮营养植物杆菌新属，sp. nov.的菌株，归属于以菌株LS 8和LS 18为代表的细菌群。模式菌株为LS 8（T）（=DSM 17806（T）= LMG 23328（T）= CGMCC 1.5339（T））。菌株LS 8（T）的DNA G+C含量为58.6 +/-0.5mol%。
Twenty-three nitrogen-fixing bacteria were isolated from surface-sterilized stems and roots of wild rice Oryza rufipogon. Four clusters were defined among these bacteria by SDS-PAGE protein patterns and further confirmed by IS-PCR finger-printing analysis. Phylogenetic analysis of 16S rRNA gene sequences showed that the representative strains LS 8 and LS 18 of cluster II formed a monophyletic group sharing 94.0-97.3% similarities with defined enterobacterial species within the genera Salmonella, Citrobacter, Pantoea, Klebsiella, and Enterobacter. DNA-DNA hybridization, physiological, biochemical tests, and cell morphology also revealed that these strains could be differentiated from the related enterobacterial species. Based upon these results, we propose Phytobacter diazotrophicus gen. nov., sp. nov. to the bacterial group represented by strains LS 8 and LS 18. The type strain is LS 8(T) (=DSM 17806(T) = LMG 23328(T) = CGMCC 1.5339(T)). The DNA G+C content of strain LS 8(T) is 58.6 +/- 0.5 mol%.