A functional and structural analysis of the Sex combs reduced locus of Drosophila melanogaster.
A functional and structural analysis of the Sex combs reduced locus of Drosophila melanogaster.
复制标题
DOI：
--
复制
发表时间：
1991-10
期刊：
影响因子：
3.3
通讯作者：
Angela;-M.;Pattatucci;D. Otteson;T. Kaufman
中科院分区：
文献类型：
--
作者：
Angela;-M.;Pattatucci;D. Otteson;T. Kaufman
我们已经进行了发育遗传分析的同源异型基因性梳减少（Scr）的果蝇通过检查胚胎和成人表型的突变影响Scr基因功能。Scr断裂点损伤的分子定位已经确定了一段大于70 kb的DNA，这是Scr基因正常功能所必需的。该区域被fushi tarazu（ftz）基因分裂，影响胚胎Scr功能的病变分子定位于ftz近端（5 '）区域，而表现出多相半致死性的病变主要定位于ftz远端（3'）。功能获得性突变与基因组重排和整个Scr基因座的图谱相关。我们的分析表明，Scr基因座包括遗传元件，负责在胚胎和幼虫到成人的发展时期的功能。从这些研究中，我们得出结论，Scr是一个复杂的遗传位点，具有广泛的调控区域，指导胚胎和成人正常头部和胸部发育所需的功能，并且在这两个时期Scr的调控是不同的。
We have undertaken a developmental genetic analysis of the homeotic gene Sex combs reduced (Scr) of Drosophila melanogaster by examining embryonic and adult phenotypes of mutations affecting Scr gene function. Molecular mapping of Scr breakpoint lesions has defined a segment of greater than 70 kb of DNA necessary for proper Scr gene function. This region is split by the fushi tarazu (ftz) gene, with lesions affecting embryonic Scr function molecularly mapping to the region proximal (5') to ftz and those exhibiting polyphasic semilethality predominantly mapping distal (3') to ftz. Gain-of-function mutations are associated with genomic rearrangements and map throughout the Scr locus. Our analysis has revealed that the Scr locus encompasses genetic elements that are responsible for functions in both the embryonic and larval to adult periods of development. From these studies, we conclude that Scr is a complex genetic locus with an extensive regulatory region that directs functions required for normal head and thoracic development in both the embryo and the adult and that the regulation of Scr during these two periods is distinct.