Distinct modulation of allergic T cell responses by subcutaneous vs. sublingual allergen-specific immunotherapy.
Distinct modulation of allergic T cell responses by subcutaneous vs. sublingual allergen-specific immunotherapy.
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DOI：
10.1111/cea.12653
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发表时间：
2016-03
期刊：
影响因子：
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通讯作者：
Peters B
中科院分区：
文献类型：
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作者：
Schulten V;Tripple V;Aasbjerg K;Backer V;Lund G;Würtzen PA;Sette A;Peters B
Allergen-specific immunotherapy is the only curative treatment for type I allergy. It can be administered subcutaneously (SCIT) or sublingually (SLIT). The clinical efficacy of these two treatment modalities appears to be similar, but potential differences in the immunological mechanisms involved have not been fully explored. To compare changes in the allergen-specific T cell response induced by subcutaneous versus sublingual administration of allergen-specific immunotherapy (AIT). Grass pollen allergic patients were randomized into groups receiving either SCIT injections, or SLIT tablets or neither. PBMC were tested for Timothy grass (TG)-specific cytokine production by ELISPOT after in vitro expansion with TG peptide pools. Phenotypic characterization of cytokine producing cells was performed by FACS. In the SCIT group, decreased IL-5 production was observed starting 10 months after treatment was commenced. At 24 months, T cell responses showed IL-5 levels significantly below the before treatment baseline. No significant reduction of IL-5 was observed in the SLIT or untreated group. However, a significant transient increase in IL-10 production after 10 months of treatment compared to baseline was detected in both treatment groups. FACS analysis revealed that IL-10 production was associated with CD4+ T cells that also produced IFNγ, and therefore may be associated with an IL-10-secreting type 1 cell phenotype. The most dominant immunological changes on a cellular level was a decrease in IL-5 in the SCIT group and a significant, transient increase of IL-10 observed after 10 months of treatment in both treated groups. The distinct routes of AIT administration may induce different immune-modulatory mechanisms at the cellular level.
