ALTERATIONS IN ORGANIZATION OF PHOSPHOLIPIDS IN ERYTHROCYTES AS FACTOR IN ADHERENCE TO ENDOTHELIAL-CELLS IN DIABETES-MELLITUS
ALTERATIONS IN ORGANIZATION OF PHOSPHOLIPIDS IN ERYTHROCYTES AS FACTOR IN ADHERENCE TO ENDOTHELIAL-CELLS IN DIABETES-MELLITUS
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DOI：
10.2337/diabetes.37.1.104
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发表时间：
1988-01-01
期刊：
影响因子：
7.7
通讯作者：
KALRA, VK
中科院分区：
文献类型：
--
作者：
WALI, RK;JAFFE, S;KALRA, VK
糖尿病患者的红细胞与培养的人血管内皮细胞的粘附性增加。我们研究了红细胞表面特性的变化，这可能是导致它们异常黏附的原因。通过磷脂酶A2处理和荧光胺和三硝基苯磺酸(TNBS)化学标记，糖尿病患者红细胞中磷脂双层的组织发生了变化。具体地说，糖尿病患者红细胞(n=25)中12-18%的磷脂酰丝氨酸可被磷脂酶A2水解和TNBS标记，而正常人则没有。这些结果提示糖尿病患者体内红细胞膜的不对称性丧失或不稳定，导致磷脂酶A2降解的可及性增加。染料花菁540(MC-540)对脂类在膜双层中的堆积很敏感，它在糖尿病患者的红细胞中显示出比正常人更多的结合和荧光。流式细胞仪分析，64.5。+-。糖尿病患者中有17.0%的红细胞(RBC)，而35.1+-。25.9%的正常人红细胞MC-540结合率为阳性，表明两层膜外叶的脂质堆积有显著差异(P<；.001)。我们的研究结果表明，糖尿病红细胞膜外叶脂质不对称和/或排列不整齐的丧失可能是红细胞与血管内皮细胞黏附倾向增加的原因。
Erythrocytes from patients with diabetes mellitus exhibit increased adherence to cultured human vascular endothelial cells. We investigated the alterations in erythrocyte surface characteristics that may contribute to their abnormal adherence. The organization of phospholipids in the lipid bilayer, as determined by phospholipase A2 treatment and chemical labeling with fluorescamine and trinitrobenzene sulfonic acid (TNBS), is altered in erythrocytes from diabetic patients. Specifically, 12-18% of phosphatidylserine in diabetic erythrocytes (n = 25) is accessible to phospholipase A2 hydrolysis and TNBS labeling, compared to none in normal subjects. These results suggest either a loss in lipid assymmetry or in vivo destabilization of erythrocyte membranes in diabetic patients, causing increased accessibility to phospholipase A2 degradation. The dye merocyanine 540 (MC-540), which is sensitive to the packing of lipids in the bilayer of the membrane, revealed more binding and fluorescence in erythrocytes from diabetic patients than in those from normal subjects. On flow cytometric analysis, 64.5 .+-. 17.0% red blood cells (RBCs) in diabetic patients, compared to 35.1 .+-. 25.9% RBCs in normal subjects, showed positive MC-540 binding, indicating significant (P < .001) differences in the packing of lipids in the external leaflet of the bilayer. The results of our study suggest that a loss of lipid asymmetry and/or less ordered packing in the outer leaflet of the diabetic erythrocyte membrane may be responsible for the increased propensity of erythrocytes to adhere to vascular endothelium.