A SIMPLE CHEMOSENSORY RESPONSE IN DROSOPHILA AND THE ISOLATION OF ACJ MUTANTS IN WHICH IT IS AFFECTED
A SIMPLE CHEMOSENSORY RESPONSE IN DROSOPHILA AND THE ISOLATION OF ACJ MUTANTS IN WHICH IT IS AFFECTED
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DOI：
10.1073/pnas.86.20.8118
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发表时间：
1989-10-01
影响因子：
11.1
通讯作者：
CARLSON, J
中科院分区：
文献类型：
--
作者：
MCKENNA, M;MONTE, P;CARLSON, J
尽管果蝇视觉系统已被广泛描述，但对其嗅觉系统知之甚少。造成这种差异的一个主要原因是缺乏简单、可靠的方法来测量对空气中化学物质的反应。本文描述了将果蝇暴露于化学蒸气中引起的跳跃反应。这种行为为单蝇化学感应测定提供了基础。该行为表现出剂量依赖性和化学特异性：暴露于乙酸乙酯、苯甲醛和丙酸而不是乙醇会刺激它。动物可以在短时间内对乙酸乙酯和丙酸产生反复反应。响应依赖于第三触角节。为了说明这种行为在化学感应反应的遗传分析中的用途，分离了九个反应缺陷的 acj 突变体（acj = 异常化学感应跳跃），并表征了它们对两种化学物质的反应。所有 acj 突变体在巨纤维系统生理学中都是正常的，并且两个在视觉系统生理学中表现出缺陷。
Although the Drosophilia visual system has been described extensively, little is known about its olfactory system. A major reason for this discrepancy has been the lack of simple, reliable means of measuring response to airborne chemicals. This paper describes a jump response elicited by exposing Drosophila to chemical vapors. This behavior provides the basis for a single-fly chemosensory assay. The behavior exhibits dose dependence and chemical specificity: it is stimulated by exposure to ethyl acetate, benzaldehyde, and propionic acid but not ethanol. Animals can respond repeatedly at short intervals to ethyl acetate and propionic acid. The response relies on the third antennal segments. To illustrate the use of this behavior in genetic analysis of chemosensory response, nine acj mutants defective in response are isolated (acj = abnormal chemosensory jump), and their responses to two chemicals are characterized. All of the acj mutants are normal in giant fiber system physiology, and two exhibit defects in visual system physiology.