Further Evidence for the Presence of Mitochondrially Encoded Subunits in Cytochrome c Oxidase of the Trypanosomatid Crithidia fasciculata
Further Evidence for the Presence of Mitochondrially Encoded Subunits in Cytochrome c Oxidase of the Trypanosomatid Crithidia fasciculata
复制标题
锥虫 Crithidia fasciculata 细胞色素 c 氧化酶中存在线粒体编码亚基的进一步证据
作者：
C. K. Breek;D. Speijer;H. Dekker;A. Muijsers;R. Benne
Mitochondrial mRNAs in trypanosomatids are edited by uridylate insertion and deletion. The respiratory chain complexes cytochrome c reductase, cytochrome c oxidase and F0F1-ATPase of the insect trypanosomatid Crithidia fasciculata have been isolated and analysed by peptide microsequencing, but so far, proteins encoded by edited (and unedited) mitochondrial mRNAs have not been found. In this paper, we provide evidence that the mitochondrial mRNAs encoding the three large subunits of cytochrome c oxidase are indeed translated. First, purified holo cytochrome c oxidase turned out to be cysteine-rich, in agreement with the high cysteine codon-content of the sequence of mitochondrial cox subunit mRNAs. Second, in mass spectrometry measurements of cytochrome c oxidase, a protein was detected with the predicted molecular weight of cytochrome c oxidase subunit 2. Finally, an antibody generated against a fusion protein produced in Escherichia coli from constructs containing a segment of cytochrome c oxidase subunit 2 cDNA, specifically recognised protein bands present in cytochrome c oxidase following SDS PAGE. However, these proteins were present in the high molecular weight region of the gel, suggesting that cytochrome c oxidase subunit 2 aggregates in the presence of SDS.
从 Crithidia fasciculata 中纯化的细胞色素 c 还原酶含有非典型细胞色素 c1。
DOI：
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发表时间：
1992
期刊：
The Journal of biological chemistry
影响因子：
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作者：
Priest,JW;Hajduk,SL
通讯作者：
Hajduk,SL
影响因子：
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作者：
Roderick A. Capaldi;Stephen D. Fuller;Victor M. Darley-Usmar
通讯作者：
Roderick A. Capaldi;Stephen D. Fuller;Victor M. Darley-Usmar