Genetic characterization of penicillin-binding proteins of nonencapsulated Streptococcus pneumoniae in the post-pneumococcal conjugate vaccine era in Japan
Genetic characterization of penicillin-binding proteins of nonencapsulated Streptococcus pneumoniae in the post-pneumococcal conjugate vaccine era in Japan
复制标题
日本后肺炎球菌结合疫苗时代非包膜肺炎链球菌青霉素结合蛋白的遗传特征
DOI：
10.1016/j.ijid.2022.04.033
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发表时间：
2022
影响因子：
8.4
通讯作者：
Nobumichi Kobayashi
中科院分区：
文献类型：
--
作者：
Mitsuyo Kawaguchiya;Noriko Urushibara;Meiji Soe Aung;Kenji Kudo;Masahiko Ito;Satoshi Habadera;Nobumichi Kobayashi
目的肺炎链球菌结合疫苗（PCV）引入后，非灭活肺炎链球菌（NESP）开始出现.本研究旨在阐明青霉素结合蛋白的遗传特征（PBPs; PBP 1a，2b和2x）与紧急NESp中青霉素不敏感性相关。结果共鉴定出21个不同的PBP基因型（1a-2b-2x），所有这些都代表新的PBP谱。13-16-ne 1（32.4%，n = 23）、ne 1 -16-ne 2（14.1%，n = 10）和13-7-ne 4（7.0%，n = 5）是PBP的主要类型（新PBP类型以“ne”表示“无包膜”），占总PBP的53.5%。所有具有PBP谱13-16-ne 1和13-7-ne 4的分离株以及具有PBP 1a-13和-131型、PBP 2b-7型、-ne 1和-ne 2的分离株均显示对青霉素不敏感。高度的遗传多样性被发现在PBP 2x，其中大部分（81.7%）是新的types.ConclusionsOur目前的研究确定了21个新的PBP配置文件和显着的突变的PBPs，这可能是潜在的与青霉素非敏感性NESP。
ObjectivesNonencapsulatedStreptococcus pneumoniae(NESp) is emerging after the introduction of pneumococcal conjugate vaccines (PCVs). This study aimed to elucidate the genetic characteristics of penicillin-binding proteins (PBPs; PBP1a, 2b, and 2x) associated with penicillin nonsusceptibility in emergent NESp.MethodsA total of 71 NESp isolates that were identified in our previous study during the PCV era in Japan (2011–2019) were analyzed for their amino acid sequences of transpeptidase domain in PBP 1a, 2b, and 2x.ResultsOverall, we identified 21 different PBP profiles (1a-2b-2x), all of which represent novel PBP profiles. The dominant PBP profiles were 13-16-ne1 (32.4%,n= 23), ne1-16-ne2 (14.1%,n= 10), and 13-7-ne4 (7.0%,n= 5) (novel PBP type was numbered with “ne” denoting “nonencapsulated”), accounting for 53.5% of all isolates. All isolates with the PBP profiles 13-16-ne1 and 13-7-ne4 and those having PBP1a type-13 and -131, PBP2b type-7, -ne1, and -ne2 showed nonsusceptibility to penicillin. A high degree of genetic diversity was found in PBP2x, with most of them (81.7%) being new types.ConclusionsOur current study identified the 21 novel PBP profiles and remarkable mutations in the PBPs, which may be potentially associated with penicillin nonsusceptibility in NESp.