Microfluidic multiple cross-correlated Coulter counter for improved particle size analysis
Microfluidic multiple cross-correlated Coulter counter for improved particle size analysis
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DOI：
10.1016/j.snb.2019.05.092
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发表时间：
2019-10
期刊：
影响因子：
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通讯作者：
Wenchang Zhang;Yuan Hu;G. Choi;Shengfa Liang;Ming Liu;W. Guan
中科院分区：
文献类型：
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作者：
Wenchang Zhang;Yuan Hu;G. Choi;Shengfa Liang;Ming Liu;W. Guan
库尔特计数器（又名电阻脉冲传感器）被广泛用于测量生物细胞和胶体颗粒的尺寸。库尔特计数器的重要参数之一是其大小鉴别能力。本工作报告了一种基于多孔的微流体库尔特计数器，用于改进混合样品中的尺寸区分。当单个颗粒穿过传感孔阵列时，产生多个时间相关的电阻脉冲信号。由于这些电阻脉冲信号的时间相关性，我们发现，多重互相关分析（MCCA）可以提高尺寸的信噪比（SNR）的一个因素，n1/2，其中是孔数的序列。这种概念验证是用聚苯乙烯珠以及人红细胞进行实验验证的。我们预计这种方法将是非常有益的应用程序中需要改进的大小分化。
Coulter counters (a.k.a. resistive pulse sensors) were widely used to measure the size of biological cells and colloidal particles. One of the important parameters of Coulter counters is its size discriminative capability. This work reports a multiple pore-based microfluidic Coulter counter for improved size differentiation in a mixed sample. When a single particle translocated across an array of sensing pores, multiple time-related resistive pulse signals were generated. Due to the time correlation of these resistive pulse signals, we found a multiple cross-correlation analysis (MCCA) could enhance the sizing signal-to-noise (SNR) ratio by a factor ofn1/2, wherenis the pore numbers in series. This proof-of-concept is experimentally validated with polystyrene beads as well as human red blood cells. We anticipate this method would be highly beneficial for applications where improved size differentiation is required.