Origin of the early megakaryopoietic progenitor cell and further polyploidization in the thrombopoietic cell series. Phase-contrast observations of human bone marrow.
Origin of the early megakaryopoietic progenitor cell and further polyploidization in the thrombopoietic cell series. Phase-contrast observations of human bone marrow.
复制标题
早期巨核祖细胞的起源和血小板生成细胞系列的进一步多倍化。
DOI：
10.1159/000207118
复制
发表时间：
1981
影响因子：
2.4
通讯作者：
I. Boll
中科院分区：
文献类型：
--
作者：
I. Boll
通过相差延时显微摄影技术对活细胞进行连续观察，揭示了造血祖细胞系列中多核细胞和分叶细胞的不同起源，必须将其归类为巨核细胞，即巨核细胞系列中确定的祖细胞。具有核直径为 5-13 微米的单核的造血祖细胞，即原巨核细胞，可以通过各种方式变成多倍体：通过有丝分裂后细胞质融合、通过连续核融合、通过核内有丝分裂、或通过核无丝分裂或核内复制和核生长后的核叶形成。包括无丝分裂核分裂在内的正常祖细胞的有丝分裂率接近一半，所有这些处于G2期的细胞（0.4）在体外分别产生八倍体巨核细胞和破骨细胞。培养基中富含血小板生成素的血清将造血祖细胞系的有丝分裂指数从0.1提高至0.25，但不改变多倍体有丝分裂的比例。
Continuous observation of living cells by phase-contrast time lapse microcinematography revealed different origins of multinuclear cells and cells with lobed nuclei in the hematopoietic progenitor cell series, which must be classified as megakaryoblasts, the determined progenitor cells of the megakaryopoietic series. Hematopoietic progenitor cells with single nuclei of nuclear diameter 5-13 microns, the promegakaryoblasts, may become polyploid by various means: by postmitotic cytoplasmic fusion, by consecutive nuclear fusion, by endomitosis, or by nuclear amitosis or lobe formation presumably after endoreduplication and nuclear growth. Including amitotic nuclear division in the mitotic rate of normal progenitor cells nearly the half of all these cells in G2 phase (0.4) produce in vitro octoploid megakaryoblasts, respectively osteoclasts. Thrombopoietin-rich sera in the medium enhance the mitotic index of the hematopoietic progenitor cell line from 0.1 to 0.25, but do not change the proportion of polyploid mitoses.