Biomolecular transport through hemofiltration membranes.
Biomolecular transport through hemofiltration membranes.
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DOI：
10.1007/s10439-009-9642-0
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发表时间：
2009-04
影响因子：
3.8
通讯作者：
Roy, Shuvo
中科院分区：
文献类型：
--
作者：
Conlisk, A. T.;Datta, Subhra;Fissell, William H.;Roy, Shuvo
开发了在存在跨膜压力、施加/诱导电场以及孔入口和出口处的不同相互作用的情况下通过孔过滤大溶质的理论模型，以表征和预测具有纳米级孔的血液滤过膜的实验性能，该血液滤过膜设计用于拟定的植入式肾脏辅助装置（RAD）。该模型表明，可以通过施加外部电场，并确保一个较小的比例的孔进料和孔渗透平衡分配系数时，扩散存在的膜的筛分特性得到改善。然后定制该模型以研究RAD血液滤过装置的狭缝形纳米孔中带电和不带电溶质的筛分特性。流动电位或感应场的影响被认为是可以忽略不计的代表性的操作条件下。报道了牛血清白蛋白、碳酸酐酶和甲状腺球蛋白的筛分系数的实验数据，并与理论预测进行了比较。空间和静电分配被认为是和比较表明，在一般的静电效应存在于蛋白质的过滤，虽然一些数据，特别是那些记录在一个强高渗溶液（10×PBS），显示出更好的协议与空间分配理论。
A theoretical model for filtration of large solutes through a pore in the presence of transmembrane pressures, applied/induced electric fields, and dissimilar interactions at the pore entrance and exit is developed to characterize and predict the experimental performance of a hemofiltration membrane with nanometer scale pores designed for a proposed implantable Renal Assist Device (RAD). The model reveals that the sieving characteristics of the membrane can be improved by applying an external electric field, and ensuring a smaller ratio of the pore-feed and pore-permeate equilibrium partitioning coefficients when diffusion is present. The model is then customized to study the sieving characteristics for both charged and uncharged solutes in the slit-shaped nanopores of the hemofiltration device for the RAD. The effect of streaming potential or induced fields are found to be negligible under representative operating conditions. Experimental data on the sieving coefficient of bovine serum albumin, carbonic anhydrase and thyroglobulin are reported and compared with the theoretical predictions. Both steric and electrostatic partitioning are considered and the comparison suggests that in general electrostatic effects are present in the filtration of proteins though some data, particularly those recorded in a strongly hypertonic solution (10×PBS), show better agreement with the steric partitioning theory.
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影响因子：
--
作者：
DEEN, WM;SATVAT, B;JAMIESON, JM
通讯作者：
JAMIESON, JM
DOI：
10.1073/pnas.91.25.12027
发表时间：
1994-12-06
影响因子：
11.1
作者：
GAO, JM;GOMEZ, FA;WHITESIDES, GM
通讯作者：
WHITESIDES, GM
影响因子：
3.7
作者：
GANATOS, P;PFEFFER, R;WEINBAUM, S
通讯作者：
WEINBAUM, S
DOI：
10.1152/ajprenal.00097.2007
发表时间：
2007-10-01
影响因子：
4.2
作者：
Fissell, William H.;Manley, Sargum;Roy, Shuvo
通讯作者：
Roy, Shuvo
影响因子：
--
作者：
HOGG, R;HEALY, TW;FUERSTENAU, DW
通讯作者：
FUERSTENAU, DW