Subnetwork-Specific Homeostatic Plasticity in Mouse Visual Cortex In Vivo
Subnetwork-Specific Homeostatic Plasticity in Mouse Visual Cortex In Vivo
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DOI：
10.1016/j.neuron.2015.05.010
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发表时间：
2015-06-03
期刊：
影响因子：
16.2
通讯作者：
Keck, Tara
中科院分区：
文献类型：
--
作者：
Barnes, Samuel J.;Sammons, Rosanna P.;Keck, Tara
体内平衡调节已被证明可以在感觉剥夺后恢复体内皮质活动，但尚不清楚这种恢复是否在所有细胞中都是一致的，还是特定于网络的子集。为了解决这个问题，我们在行为成年小鼠中使用慢性钙成像来检查摘除后单眼视觉皮层 2/3 层同一区域中单个兴奋性和抑制性神经元的活动。我们发现，只有一小部分兴奋性神经元在剥夺后稳态恢复活动，而抑制性神经元则没有恢复。在剥夺之前，确实恢复的兴奋性细胞更有可能与其他恢复的兴奋性神经元具有显着相关的活动，从而形成恢复神经元的子网络。这些网络水平的变化伴随着对所有兴奋性神经元的突触抑制的减少，这表明突触机制和子网络活动对于剥夺后活动的稳态恢复很重要。
Homeostatic regulation has been shown to restore cortical activity in vivo following sensory deprivation, but it is unclear whether this recovery is uniform across all cells or specific to a subset of the network. To address this issue, we used chronic calcium imaging in behaving adult mice to examine the activity of individual excitatory and inhibitory neurons in the same region of the layer 2/3 monocular visual cortex following enucleation. We found that only a fraction of excitatory neurons homeostatically recover activity after deprivation and inhibitory neurons show no recovery. Prior to deprivation, excitatory cells that did recover were more likely to have significantly correlated activity with other recovering excitatory neurons, thus forming a subnetwork of recovering neurons. These network level changes are accompanied by a reduction in synaptic inhibition onto all excitatory neurons, suggesting that both synaptic mechanisms and subnetwork activity are important for homeostatic recovery of activity after deprivation.