Mouse Fem1b interacts with and induces ubiquitin-mediated degradation of Ankrd37
Mouse Fem1b interacts with and induces ubiquitin-mediated degradation of Ankrd37
复制标题
小鼠 Fem1b 与 Ankrd37 相互作用并诱导泛素介导的降解
DOI：
10.1016/j.gene.2011.06.025
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发表时间：
2011-10-10
期刊：
影响因子：
3.5
通讯作者：
Han, Chun-Sheng
中科院分区：
文献类型：
--
作者：
Shi, Yu-Qiang;Liao, Shang-Ying;Han, Chun-Sheng
锚蛋白重复结构域37 （Ankrd37）是一种含有锚蛋白重复序列（ARs）和假定的核定位信号（NLS）的蛋白，从斑马鱼到人类都高度保守。在小鼠睾丸中，Ankrd37蛋白最初存在于伸长精子的细胞质中，最终在精子发生过程中局限于精子的细胞核。酵母双杂交筛选和共免疫沉淀试验表明，Ankrd37与雌性化1同源物b （Fem1b）结合。在共转染的CHO细胞中，Ankrd37促进了Fem1b蛋白从细胞质到细胞核的运输。此外，转染实验显示Ankrd37的蛋白水平呈Fem1b剂量依赖性降低，并且Ankrd37在Fem1b存在下泛素化。由于线虫Fem-1已被证明靶向其下游效应物TRA-1进行泛素介导的降解，我们在本研究中报道小鼠Fem1b以相同的方式靶向Ankrd37进行降解。(C) 2011 Elsevier B.V.版权所有
Ankyrin repeat domain 37 (Ankrd37), a protein containing ankyrin repeats (ARs) and a putative nuclear localization signal (NLS), is highly conserved from zebrafish to humans. In mouse testes, Ankrd37 protein was initially present in the cytoplasm of elongating spermatids, and finally restricted to the nuclei of spermatozoa during spermatogenesis. Ankrd37 bound to feminization 1 homolog b (Fem1b) as indicated by yeast two-hybrid screening and co-immunoprecipitation assays. Ankrd37 facilitated the transport of Fem1b protein from cytoplasm to nuclei in co-transfected CHO cells. In addition, the protein level of Ankrd37 was decreased in a Fem1b dose-dependent manner as shown by the transfection experiments, and Ankrd37 was ubiquitinated in the presence of Fem1b. As the nematode Fem-1 has been shown to target its downstream effector TRA-1 for ubiquitin-mediated degradation, we report in the present study that mouse Fem1b targets Ankrd37 for degradation in the same manner. (C) 2011 Elsevier B.V. All rights reserved.