FTIR/ATR for protein adsorption to biomaterial surfaces
FTIR/ATR for protein adsorption to biomaterial surfaces
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DOI：
10.1016/s0142-9612(97)00223-8
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发表时间：
1998-03-01
期刊：
影响因子：
14
通讯作者：
Chittur, KK
中科院分区：
文献类型：
--
作者：
Chittur, KK
目前普遍认为，血液蛋白最初快速吸附到生物材料表面对植入物的长期性能很重要。与植入物相互作用的细胞将对一层（单层或多层）吸附的蛋白质发生反应。蛋白质吸附到生物材料感兴趣的表面的研究中的重要参数包括不同吸附的蛋白质的总量和这些吸附的蛋白质的构象和取向。研究人员已经开发了一些技术，我们现在可以解决所有这些问题。在本文中，我们讨论了傅里叶变换红外（FTIR）衰减全内反射（ATR）技术可以用于生物材料表面和生物材料表面的事件，如蛋白质吸附的研究。FTIR光谱比使用光栅的光谱仪具有更高的信噪比和速度，因此能够观察蛋白质与表面相互作用时的关键早期事件。也许FTIR技术相对于色散光谱仪的最大优势是波长精度。这允许从水溶液中蛋白质的光谱中减去水，一种强红外吸收剂。本文首先介绍了ATR如何用于提供表面蛋白质的信息。从ATR光谱的分析计算的蛋白质吸附到表面的量的方程。讨论了可以用FTIR/ATR分析的表面种类和扣除H2O的困难。评论的其余部分涉及如何感兴趣的生物材料研究人员，如蛋白质吸附动力学，蛋白质二级结构的变化和吸附到表面上的方向可以通过FTIR/ATR获得的信息。(C)1998由Elsevier Science Ltd.出版。保留所有权利。
It is now well accepted that the initial rapid adsorption of blood proteins to biomaterial surfaces is important in the long-term performance of the implant. Cells that interact with the implant will be reacting to a layer (single or multiple) of adsorbed protein. The parameters of importance in a study of protein adsorption to surfaces of biomaterial interest include total amounts of different adsorbed proteins and the conformation and orientation of these adsorbed proteins. Researchers have developed a number of techniques with which we can now address all these questions. In this paper, we have discussed how Fourier transform infrared (FTIR) attenuated total internal reflection (ATR) techniques can be used for the study of biomaterial surfaces and events at biomaterial surfaces such as protein adsorption. FTIR spectroscopy offers higher signal-to-noise and speeds than spectrometers that use gratings and hence offers the capability of observing the critical early events when proteins interact with surfaces. Perhaps the biggest advantage of the FTIR technique over dispersive spectrometers is wavelength precision. This allows the subtraction of water, a strong infrared absorber, from the spectra of proteins in aqueous solutions. This review starts with an introduction of how ATR can be used to provide information about proteins on surfaces. Equations to calculate the amount of proteins adsorbed to surfaces from analysis of ATR spectra are presented. A discussion of the kinds of surfaces that can be analyzed by FTIR/ATR and difficulties with the subtraction of H2O is given. The rest of the review deals with how information of interest to biomaterials researchers such as kinetics of protein adsorption, changes in protein secondary structure and orientation upon adsorption to surfaces can be obtained by FTIR/ATR. (C) 1998 Published by Elsevier Science Ltd. All rights reserved.